• European Marine Science

Entretien avec les descendants d'un anthropologue maritime au sujet de l'Arctique et de ses missions ethnographiques.

Abstract Pakistan is an agricultural country and fisheries play a very important role in the economic development of the country. Different diseases are prevalent in Pakistani fish but information related to the causative agents is not well-known. Keeping in view the significance of bacterial pathogens as the causative agents of multiple fish diseases, the present study was conducted for identification, characterization and analysis of virulence genes of Aeromonas spp. isolated from diseased fishes. A total of fifty fish samples having multiple clinical indications were collected from different fish farms of district Kasur, Punjab Pakistan. For isolation of Aeromonas spp. samples were enriched and inoculated on Aeromonas isolation medium. Isolates were identified and characterized by different biochemical tests, Analytical Profile Index (API) 20E kit and Polymerase Chain Reaction (PCR) assays. All isolates were screened for three putative virulence genes including aerolysin (aer), haemolysin (hyl) and heat labile cytotonic enterotoxin (alt). Seven isolates of Aeromonas (A.) hydrophila were retrieved and identified based on API 20E. These isolates were further confirmed as A. hydrophila on the basis of PCR assays. Three isolates were detected positive for the presence of virulence genes (alt and hyl). Whereas aerolysin (aer) gene was not present in any of A. hydrophila isolates. The present study confirmed A. hydrophila as the causative agent of epizootic ulcerative syndrome and motile Aeromonas septicemia in fish farms of district Kasur, Punjab Pakistan. Moreover, detection of two virulence genes (alt and hyl) in A. hydrophila isolates is a threat for fish consumers of study area. Resumo O Paquistão é um país agrícola, onde a pesca desempenha um papel muito importante para o desenvolvimento econômico. Diferentes doenças são prevalentes em peixes do Paquistão, mas as informações relacionadas aos agentes causadores não são bem conhecidas. Tendo em vista a importância dos patógenos bacterianos como agentes causadores de múltiplas doenças em peixes, o presente estudo foi conduzido para identificação, caracterização e análise de genes de virulência de isolados de Aeromonas spp. de peixes doentes. Foram coletadas 50 amostras de peixes com múltiplas indicações clínicas em diferentes fazendas do distrito de Kasur, Punjab, Paquistão. Para isolar Aeromonas spp., as amostras foram enriquecidas e inoculadas em meio de isolamento. Os isolados foram identificados e caracterizados por diferentes testes bioquímicos, kit Analytical Profile Index (API) 20E, e ensaios de reação em cadeia da polimerase (PCR). Todos os isolados foram selecionados para três genes de virulência putativos, incluindo aerolisina (aer), hemolisina (hyl) e enterotoxina citotônica termolábil (alt). Sete isolados de Aeromonas hydrophila foram recuperados e identificados com base no API 20E. Esses isolados foram posteriormente confirmados como A. hydrophila de acordo com ensaios de PCR. Três isolados indicaram a presença de genes de virulência (alt e hyl), enquanto o gene aerolisina (aer) não esteve presente em nenhum dos isolados de A. hydrophila. O presente estudo confirmou A. hydrophila como o agente causador da síndrome ulcerativa epizoótica e septicemia móvel por Aeromonas em fazendas de peixes, no distrito de Kasur, Punjab, Paquistão. Além disso, a detecção de dois genes de virulência (alt e hyl) em isolados de A. hydrophila é uma ameaça para os consumidores de peixes da área de estudo.

Coastal nature-based solutions (NbS) are increasingly recognized for their multiple benefits to socio-ecological systems, including climate mitigation and adaptation (e.g. conservation, restoration and sustainable management of coastal ecosystems for climate). National climate plans, such as the Nationally Determined Contributions (NDCs) developed under the Paris Agreement, include coastal NbS as a practical and effective action to help countries achieve their climate and biodiversity targets. However, the absence of a standardized NDC structure and the lack of guidance about how NbS should be included in NDCs can hinder access to external funding for developing countries and prevent transparent reporting on progress at the international level. In this context, our aim is to understand how coastal NbS are currently included in NDCs by evaluating their alignment with the IUCN Global Standard for NbS. Our analysis focuses on the description of coastal NbS in the NDCs of Pacific Small Island Developing States (PSIDS), as they are among the most vulnerable countries to the impacts of climate change. Overall, we find that, for the 22 coastal NbS examined in the NDCs of PSIDS, the degree of alignment with the eight criteria of the IUCN Global Standard is insufficient or partial, with slightly better alignment with the standard in revised NDCs than in original NDCs. We discuss opportunities provided by the standardization of the description of coastal NbS in NDCs, in terms of access to funding and stock taking to monitor the effectiveness of implementation and progress towards long-term goals. We also discuss the relevance of using the IUCN Global Standard for reporting on NbS in NDCs for PSIDS.

Ocean acidification (OA) has been identified as one of the major climate-change related threats, mainly due to its significant impacts on marine calcifiers. Among those are the calcareous green algae of the genus Halimeda that are known to be major carbonate producers in shallow tropical and subtropical seas. Hence, any negative OA impacts on these organisms may translate into significant declines in regional and global carbonate production. In this study, we compiled the available information regarding Halimeda spp. responses to OA (experimental, in situ), with special focus on the calcification responses, one of the most studied response parameters in this group. Furthermore, among the compiled studies (n = 31), we selected those reporting quantitative data of OA effects on algal net calcification in an attempt to identify potential general patterns of species- and/or regional-specific OA responses and hence, impacts on carbonate production. While obtaining general patterns was largely hampered by the often scarce number of studies on individual species and/or regions, the currently available information indicates species-specific susceptibility to OA, seemingly unrelated to evolutionary lineages (and associated differences in morphology), that is often accompanied by differences in a species� response across different regions. Thus, for projections of future declines in Halimeda-associated carbonate production, we used available regional reports of species-specific carbonate production in conjunction with experimental OA responses for the respective species and regions. Based on the available information, declines can be expected worldwide, though some regions harbouring more sensitive species might be more impacted than others.

Despite the importance of marine megafauna on ecosystem functioning, their contribution to the oceanic carbon cycle is still poorly known. Here, we explored the role of baleen whales in the biological carbon pump across the southern hemisphere based on the historical and forecasted abundance of five baleen whale species. We modelled whale-mediated carbon sequestration through the sinking of their carcasses after natural death. We provide the first temporal dynamics of this carbon pump from 1890 to 2100, considering both the effects of exploitation and climate change on whale populations. We reveal that at their pre-exploitation abundance, the five species of southern whales could sequester 4.0 × 10 5 tonnes of carbon per year (tC yr −1 ). This estimate dropped to 0.6 × 10 5 tC yr −1 by 1972 following commercial whaling. However, with the projected restoration of whale populations under a RCP8.5 climate scenario, the sequestration would reach 1.7 × 10 5 tC yr −1 by 2100, while without climate change, recovered whale populations could sequester nearly twice as much (3.2 × 10 5 tC yr −1 ) by 2100. This highlights the persistence of whaling damages on whale populations and associated services as well as the predicted harmful impacts of climate change on whale ecosystem services.

We collected specimens of Ulva sp. (green algae), Saccharina sp. (brown algae), Grateloupia sp., and Gelidium sp. (both red algae) during all four seasons from a coastal reef in Weihai, China, and analyzed their surface-colonizing bacteria in comparison to surrounding seawater and sediment controls. 16S rRNA amplicon sequencing, extensive plate cultivation, sequencing of selected strains, and sequencing of 23 metagenomes were performed in this study. Besides composition, we analyzed the potential of phycosphere bacteria to degrade algal polysaccharides and to produce bioactive secondary metabolites. This comprehensive dataset represents a significant stepping stone towards a better understanding of macroalgal phycosphere bacteria and paves the way to functional studies on representative strains.

Compact and efficient power converter solutions are seen to be the backbone of future transportation systems in order to cope with the ongoing transition toward greener systems. Such systems usually comprise a main load section, in which one or more propulsion or traction motors are connected, in addition to an auxiliary load, which might comprise the hotels and air conditioning for example. This auxiliary load can be as low as 5-10% of the main load power. Therefore, it can be challenging to drive this power from a typical high-power system that employs a medium-voltage (MV) dc (MVDC) grid, which is typical in high-power systems. In such MVDC-integrated systems, neutral-point-clamped and active neutral-point-clamped (ANPC) converters are commonly used, where the auxiliary load converter is overrated in this case, resulting in a bulky and inefficient power system. Thus, in order to enable a lighter and efficient transportation power system, a multiport hybrid converter (MHC) is presented in this article. This converter can feed the main MV motor, in addition to two auxiliary low-voltage loads. Compared with the state-of-the-art ANPC converter, the proposed MHC utilizes only two extra switches per phase leg in order to achieve this multiport operation along with increasing the voltage rating of another two switches. The proposed MHC is analyzed in this article, where its operation, modulation, and mathematical derivation are presented. These analyses are supported by simulation and experimental results utilizing a reduced-scale 5-kW system.

Coral reefs are complex habitats that contain very high biodiversity and provide different ecosystem services. In the Coral Triangle, however, various major benthic components are still understudied. This can limit our understanding of coral reef community dynamics, especially in the presence of a changing climate coupled with local disturbances (e.g., decreased water quality). This study describes the benthic community structure of an ecologically and economically important coral reef system in the central Philippines through characterizing the assemblages of three major components (hard corals, octocorals, and sponges) among sites and stations with varying environmental conditions (i.e., exposure to monsoons, water quality levels). Results reveal significant variations in the mean percentage covers of hard corals, octocorals, and sponges at the site and station levels (ANOVA, p < 0.05), with hard corals dominating in Site 1, which is more exposed to the southwest monsoon, and Site 3, which is an embayed and unexposed site with low water quality, while soft corals dominated in Site 2, which is more exposed to the northeast monsoon. Multivariate analyses also revealed significant variations in the benthic community structure at different spatial scales (ANOSIM, p < 0.05). Interestingly, even stations within a site had significant variations in community structure, with different taxa being dominant. This study highlights the importance of conducting more detailed analyses of understudied taxa (i.e., octocorals and sponges) during coral reef surveys to improve our understanding of coral reef community dynamics that is very important for management.

The sustainable development of the aquaculture industry relies on the use of alternative conventional and emergent raw materials that contributes to a circular economy and to reduce the dependency on fish meals and fish oils coming from oceanic fish populations. Additionally, the genetic selection of farmed fish that can display higher growth and feed utilization when fed alternative feeds, is pointed out to be a complementary valuable tool to facilitate the implementation of circular economy approaches. The main purpose of the present study was to determine the effectiveness of genetic selection for growth in European sea bass, in response to a challenge with an alternative diet that aimed to partially replaced fishmeal (FM) by poultry meal (PM) and totally replace fish oil (FO) by a blend of poultry oil (PO) with a novel microalgae oil. The two families of fish juveniles were obtained by in vitro fertilization of selected for a multi-trait including high growth (genetically selected, GS) or non-selected (wild type, WT) broodstocks and then were nutritionally challenged with a control diet that mirrored a standard commercial diet with fishmeal (20%) and fish oil (7%), or a Future diet that partially replaced the FM by PM and totally replaced the FO by a blend of rapeseed oil, PO, and a novel DHA rich-algal oil. From the second month of feeding until the end of the trial, European sea bass that was selected since for 7 generations performed better in terms of growth than the wild-type genotype, possibly related with an apparent favored feed and nutrient utilization. Furthermore, selection decreased the perivisceral fat and increased the nutritional value of flesh by increasing DHA (in g/ 100 g flesh) and ARA contents. In contrast, the dietary treatment showed little effect on fish growth performance, denoting the successful partial replacement of FM by PM and the total replacement of FO by a blend of poultry oil and an emergent microalgal oil. However, Future diet tended to reduce the ADCs of some amino acids, as well as showed an additive effect to genotype in increasing the n-3 PUFA of flesh. Altogether, our data demonstrate that multi-trait genetic selection of European sea bass improve fish plasticity to cope with the variations of ingredients in alternative feeds with low FM/FO.

Most continental arcs are built up over a long time (≥100 myr), and while subduction may be ongoing throughout this interval, magmatism appears to be highly episodic. This episodic behaviour is characterized by high-flux magmatic events but an overall low rate of magmatism. The causes of high-flux magmatic events (“flare-ups”) are enigmatic in many continental arcs. Bulk-rock Sr, Nd, and Pb isotopes, as well as zircon O and Hf isotopes, imply that the mantle and the continental crust can be involved in magmatic flare-ups. However, the relative contributions of mantle vs. crust with changes in eruption rates can differ from arc to arc. The Cenozoic magmatic arcs of Iran, built on mature continental crust, are an excellent candidate for studying the geochemical-isotopic feedback of magmatic pulses to understand the triggers for a flare-up. Our new data constrain the timing of the flare-up in NE Iran to the Early to Middle Eocene (51–43 Ma). This flare-up is characterized by the outpouring of high-K calc-alkalic to shoshonitic magmas at ∼110 ± 8 km3/myr - km. Geochemical modelling using the “Arc Basalt Simulator version 3″ shows that the high-K trachybasalts, moderately to extremely depleted in high-field strength elements, can be derived from the shallower (3.0 GPa; 870 °C) to deeper parts (5.0–5.4 GPa; 965–980 °C) of a subducting slab with ∼1.0 to 5.5 % slab melt flux. Mixing modelling using Sr, Nd, and Pb isotope data indicates that the Torud mafic-intermediate magmatic rocks can be generated by adding ∼ 1% to <6% of slab components (50% AOC: 50% sediment) to an Indian MORB-like mantle. Our results suggest that the high magmatic fluxes in NE Iran were instigated mainly by Eocene slab steepening after Paleocene flat-slab subduction, resulting in enhanced upwelling and melting of a volatile-enriched asthenospheric mantle.