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25 Research products, page 2 of 3

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  • National Library of Serbia - Digital Object Identifier Repository

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  • Open Access English
    Authors: 
    Đokić Lidija; Savić M.; Narančić Tanja; Vasiljević Branka;
    Publisher: Archives of Biological Sciences
    Country: Serbia

    Ramonda serbica and Ramonda nathaliae, rare resurrection plants growing in the Balkan Peninsula, produce a high amount of phenolic compounds as a response to stress. The composition and size of bacterial communities in two rhizosphere soil samples of these plants were analyzed using a metagenomic approach. Fluorescent in situ hybridization (FISH) experiments together with DAPI staining showed that the metabolically active bacteria represent only a small fraction, approximately 5%, of total soil bacteria. Using universal bacteria - specific primers 16S rDNA genes were amplified directly from metagenomic DNAs and two libraries were constructed. The Restriction Fragment Length Polymorphism (RLFP) method was used in library screening. Amongst 192 clones, 35 unique operational taxonomic units (OTUs) were determined from the rhizosphere of R. nathaliae, and 13 OTUs out of 80 clones in total from the library of R. serbica. Representative clones from each OTU were sequenced. The majority of sequences from metagenomes showed very little similarity to any cultured bacteria. In conclusion, the bacterial communities in the studied soil samples showed quite poor diversity. .

  • Open Access English
    Authors: 
    Relić Renata R.; Hristov Slavča V.; Vučinić Marijana M.; Poleksić Vesna D.; Marković Zoran Z.;
    Publisher: Journal of Agricultural Sciences, Belgrade
    Country: Serbia

    For several decades fish welfare has been subject of many researches, and the interest for this subject is connected with the fact that fish can feel pain and suffering. In addition to this stressors’ mechanisms of action and their consequences are similar in mammals and fish. Assessment of welfare for farmed fish is based on the same principles as for terrestrial farm animals. However, special methods of collecting data are needed due to morphological characteristics of fish and properties of their environment. In the world and in our country, researches of different ways of fish welfare assessment are being conducted, especially based on non-invasive techniques such as monitoring of behaviour. In this paper a review of basic principles and methods used in assessment of farmed fish welfare is given.

  • Open Access English
    Authors: 
    Mihailović Mirjana; Petrović Miodrag; Grdović Nevena; Dinić Svetlana; Uskoković Aleksandra; Vidaković Melita; Grigorov Ilijana; Bogojević Desanka; Ivanović-Matić Svetlana; Martinović Vesna; +3 more
    Publisher: Journal of the Serbian Chemical Society
    Country: Serbia

    The enzyme CYP1A is an established biomarker polychlorinated biphenyls of fish exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The metallothioneins (MT), a family of Cys-rich proteins, bind a wide range of metals and participate in their metabolism. The aim of the study was to examine the correlation between CYP1A and MT expression in commercially important fish species Mullus barbatus and Merluccius merluccius and contaminants (PAHs, PCBs, toxic metals) in seawater and sediment from three localities with different level of contamination in the Adriatic Sea in winter, i.e., Platamuni, Valdanos and the port of Bar. The relative concentration of CYP1A was the highest in both fish species from Bar. Increased concentrations of PCBs in the seawater were observed only in Bar. A species-specific higher increase in the protein concentration of CYP1A was observed in Mullus barbatus compared to Merluccius merluccius. The levels of MT were the highest in Merluccius merluccius from Bar and in Mullus barbatus from Valdanos. The induction of MT correlated with the elevated concentrations of Cu and Pb determined by chemical analysis of the seawater from Bar and Valdanos, respectively. According to the chemical analysis of the seawater and the biological response of the fish, the Platamuni locality exhibited the lowest level of contamination.

  • Open Access English
    Authors: 
    Vučinić Marijana; Radisavljević Katarina;
    Publisher: Veterinarski glasnik
    Country: Serbia

    Teleost fish possess similar nociceptive processing systems to those found in terrestrial vertebrates. It means that they react to potential painful stimuli in a similar manner as mammals and birds. However, the welfare of fish has been the focus of less research than that of higher vertebrates. Humans may affect the welfare of fish through fisheries, aquaculture and a number of other activities. There is scientific evidence to support the assumption that fish have the capacity to experience pain because they possess functional nociceptors, endogenous opioids and opioid receptors, brain structures involved in pain processing and pathways leading from nociceptors to higher brain structures. Also, it is well documented that some anaesthetics and analgesics may reduce nociceptive responses in fish. Behavioural indicators in fish such as lip-rubbing and rocking behaviours are the best proof that fish react to potential painful stimuli. This paper is an overview of some scientific evidence on fish capacity to experience pain.

  • Open Access English
    Authors: 
    Višnjić-Jeftić Željka; Lenhardt Mirjana; Navodaru I.; Hegediš A.; Gačić Z.; Nikčević M.;
    Publisher: Archives of Biological Sciences
    Country: Serbia

    Specimens of Pontic shad (Alosa pontica Eichwald, 1838) were collected during April and May of 2006 in the Danube River (rkm 863) just downstream of the Iron Gate (Djerdap) II dam. Twenty-eight scales and 30 vertebrae were prepared and given to five interpreters with fish aging experience of from 3 to 30 years and with shad aging experience of from 0 to 12 years. Interpreters with experience in shad age determination showed lower values for the index of average percent error (IAPE) and an index of coefficient of variation (ICV). In regard to within-interpreter reproducibility, no significant differences were found between scale and vertebrae as structure for age determination. The most experienced reader showed the lowest value for IAPE, while the least experienced reader showed the highest value for IAPE. The results of this work indicate that experience in age determination by particular structure had more impact on age determination precision than specificity of structure, scale or vertebra. More work is needed on adopting standard protocols that must include some sort of common interaction between responsible age readers.

  • Open Access English
    Authors: 
    Gačić Z.; Maximova Elena; Damjanović I.; Maximov P.; Kasparson Anna; Maximov V.;
    Publisher: Archives of Biological Sciences
    Country: Serbia
  • Open Access English
    Authors: 
    Baltić Milan Ž.; Kilibarda Nataša; Teodorović Vlado; Dimitrijević Mirjana; Karabasil Neđeljko; Dokmanović Marija;
    Publisher: Veterinarski glasnik
    Country: Serbia

    The Hazard Analysis and Critical Control Point (HACCP) system is scientifically based and focused on problem prevention in order to assure the produced food products are safe to consume. Prerequisite programs such as GMP (Good Manufacturing Practices), GHP (Good Hygienic Practices) are an essential foundation for the development and implementation of successful HACCP plans. One of the preliminary tasks in the development of HACCP plan is to conduct a hazard analysis. The process of conducting a hazard analysis involves two stages. The first is hazard identification and the second stage is the HACCP team decision which potential hazards must be addressed in the HACCP plan. By definition, the HACCP concept covers all types of potential food safety hazards: biological, chemical and physical, whether they are naturally occurring in the food, contributed by the environment or generated by a mistake in the manufacturing process. In raw fish processing, potential significant biological hazards which are reasonably likely to cause illness of humans are parasites (Trematodae, Nematodae, Cestodae), bacteria (Salmonella, E. coli, Vibrio parahemolyticus, Vibrio vulnificus, Listeria monocytogenes, Clostridium botulinum, Staphyloccocus aureus), viruses (Norwalk virus, Entero virusesi, Hepatitis A, Rotovirus) and bio-toxins. Upon completion of hazard analysis, any measure(s) that are used to control the hazard(s) should be described.

  • Open Access English
    Authors: 
    Denčić-Fekete Marija; Antić D.; Davidović-Mrsić Sanja; Franić Ivana; Kraguljac-Kurtović Nada; Bila Jelena; Elezović I.;
    Publisher: Archives of Biological Sciences
    Country: Serbia

    We describe two patients with diagnosis of chronic lymphocytic leukemia (CLL) in whom interphase fluo-rescence in situ hybridization (FISH) analysis revealed trisomy 12 and del(13)(q14.3) occurring in the same clone. These abnormalities are rarely seen together and the prognostic relevance of their coexistence is still unclear. According to some data, a probable adverse prognosis for this group of patients is suggested. Our patients have been in a stable phase of the disease for more than one year since the given abnormalities were documented in their karyotypes. Further study is necessary to determine the prognostic significance of coexistence of these abnormalities in CLL patients.

  • Open Access English
    Authors: 
    Cakić Predrag; Paunović M.; Stojanović Bojana; Đikanović Vesna; Kulišić Z.;
    Publisher: Acta veterinaria
    Country: Serbia

    During the study of fish parasites in the Serbian section of the Danube River in 2003, a new Trematoda species for ichthyofauna of Serbia has been detected. It is, at the same time, the first finding of larvae of Metagonimus yokogawai in Serbia, as adult forms have been observed from mallard Anas platyrhynchus (L.), previously. Metacercariae of M. yokogawai have been identified on the scales of chub Leuciscus cephalus (L.), barbel Barbus barbus (L.), bleak Alburnus alburnus (L.), pikeperch Sander lucioperca (L.) and rudd Scardinius erythrophthalmus (L.) in the Serbian part of Danube River. The particular risk, beside the possible negative influence of spreading on fish and other host populations (some aquatic snails, fish-eating birds and some mammals), is the potential spreading of the parasitic disease in humans - Metagonimosis.

  • Open Access English
    Authors: 
    Drakulić Danijela; Nikčević Gordana; Đorđević Vesna; Stevanović Milena;
    Publisher: Archives of Biological Sciences
    Country: Serbia

    Fluorescent in situ hybridization (FISH) has become a widespread technique applicable in basic science and diagnostics. Chromosome painting represents a special application of FISH that has found increasing use in identification of complex chromosome rearrangements. Here we present a version of the Alu-PCR method modified to generate a whole chromosome painting probe (WCP) for human chromosome 19 using monochromosomal cell hybrids. In setting up conditions for this method, we established a cheap and fast approach to generation of WCPs for other human chromosomes that could be particularly useful for unambiguous identification of complex chromosomal rearrangements associated with cancer. .