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including iodothyronine deiodinases 1, 2 and 3 (dio1, dio2, dio3) was unaffected. Feeding Se enriched rotifers effectively increased Se in ABT larvae tissue. The improved growth observed in Se supplemented treatments might be related to an accelerated development as the flexion index was significantly higher in all Se enriched treatments compared to the non-supplemented control. A similar effect by Se supplementation has been previously described in Senegalese sole (Solea senegalensis) in relation to an enhanced thyroid hormone activity by Se supplementation (Ribeiro et al., 2012). The Se level of 0.10 µg g-1 dw measured in non-supplemented rotifers is below known requirements in fish (Antony Jesu Prabhu et al. 2016). In contrast, rotifers supplemented with the lowest Se level (Se3) contained 4.42 µg Se g-1, which might be sufficient to cover requirements for this mineral as selenoproteins displayed maximum expression in ABT larvae fed this treatment. The increased seleno-enzyme production might have contributed towards an improved antioxidant status in ABT larvae, indicated by a transcriptional downregulation of redox sensitive antioxidant enzymes cat and sod. In conclusion, rotifers without Se enrichment are suboptimal for ABT larvae at first feeding. A dietary Se level of 4.42 µg g-1 dw is recommended as it boosted growth performance and improved the antioxidant status in ABT larvae.

Accurate methods for assessing triploidy are important because treatments designed to induce poliploidy are not 100% and offspring from treated eggs are usually a mixture of triploids and diploids. High-quality histograms were obtained from turbot larvae and the very good reproductibility provided unambigous discrimination of diploids and triploids even in early stages development. This paper describes a rapid and simple method for triploidy determination from the larval stage of turbot (Scopthalmus maximus) by flow cytometry. Flow cytometry was more recently applied to triploidy determination and usually carried out using erythrocytes. No

This paper is an introduction of the Fisheries Research special issue on egg production methods (EPM) that emerged from a dedicated workshop held in Athens. Greece, in 2010. The workshop considered if EPMs are still valid today, it reviewed recent developments in the methods and discussed the utility of EPMs in the future. Importantly, experts from both the daily and the annual egg production methods took part in the workshop. This introduction provides the main concepts underlying EPMs. It also gives a brief history of EPM development over the last two decades with examples of their use worldwide. It provides a review on whether EPMs have fulfilled their objectives. Given their increased utilisation on new fish stocks, and their contribution to scientific advances, EPMs continue to be useful. However, concerns about the bias and precision of the methods remain, and a continuous effort to improve those issues together with the cost-efficiency of the methods is still required. (C) 2012 Published by Elsevier B.V.