In solid cancers amplified genes are potential targets of molecular therapies. In this study the 16 genes and 22 genes reportedly amplified in gastric and mammary cancers were examined by multiplex ligation-dependent probe amplification and fluorescence in situ hybridiz...

Genome-wide copy number analysis in human gastric carcinoma identifed an amplified gene locus including vascular endothelial growth factor A (VEGFA). VEGFA gene amplification was validated by fluorescence in situ hybridization assay. The levels of VEGFA gene amplificati...

15q11-q13領域はゲノム刷り込み遺伝子クラスターであり,PWS-ICを中心とした染色体ドメインレベルの転写制御の存在が明らかになっている。このPWS-IC近傍からは父方アレル特異的な長鎖ncRNA(UBE3A-ATS)が転写され,染色体ドメインレベルの転写制御の一役を担っていると考えられている。そこで,本研究ではヒト染色体工学技術を用いてPWS-ICのノックアウトを行い,UBE3A-ATSによる遺伝子発現制御機構を解析した。前年度,PWS-IC欠失染色体でUBE3A-ATSの発現を解析したところ,SNRPN,HBII-85を含め...

Studies of chromosome were conducted to progress breeding of pear through genome studies. The results are as follows; (1) Preparation of good chromosome sample using enzymatic maceration and air drying method was established, (2) The chromosome configuration was elucida...

Gene amplification is a major type of genetic aberration found in human cancers of solid types.　The amplified genes so far have been useful targets or will be potential targets for molecular therapies. In this study, cancers of the stomach, breast and colon, which are f...

By combined analysis of immunohistochemistry and fluorescence in situ hybridization (FISH) on 475 formalin-fixed paraffin-embedded tissue, 51 gastric adenocarcinoma with HER2-amplified cancer cells. The heterogeneity of HER2 amplification, if defined as the existence of...

(1) Thermal Dome in the Atmospheric Surface Layer (Nagasaki)

(1) Thermal Dome in the Atmospheric Surface Layer (Nagasaki)

(1) Thermal Dome in the Atmospheric Surface Layer (Nagasaki)