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The paper describes an illness of one-year rainbow trout fry that was characterized by gastroenteritis and progressively high mortality, but which did not indicate a typical bacterial infection; and a clinical illness of cyprinids that indicated typical acute bacterial septicemia caused by Gram-negative bacteria. These diseases of rainbow trout and cyprinids were caused by the Gram-negative motile bacterium Citrobacter freundii. Cultures of Citrobacter freundii were isolated and identified on the basis of key phenotypic characters and with the aid of the Api 20 E system. Pathohistological examination confirmed inflammatory changes in the intestine of rainbow trout; and inflammatory and necrotic changes in the internal organs of cyprinids. We were able to reproduce the illness by means of artificial infection with a pure culture of Citrobacter freundii. This is the first published report confirming Citrobacter freundii as a cause of fish disease in Serbia.

The Hazard Analysis and Critical Control Point (HACCP) system is scientifically based and focused on problem prevention in order to assure the produced food products are safe to consume. Prerequisite programs such as GMP (Good Manufacturing Practices), GHP (Good Hygienic Practices) are an essential foundation for the development and implementation of successful HACCP plans. One of the preliminary tasks in the development of HACCP plan is to conduct a hazard analysis. The process of conducting a hazard analysis involves two stages. The first is hazard identification and the second stage is the HACCP team decision which potential hazards must be addressed in the HACCP plan. By definition, the HACCP concept covers all types of potential food safety hazards: biological, chemical and physical, whether they are naturally occurring in the food, contributed by the environment or generated by a mistake in the manufacturing process. In raw fish processing, potential significant biological hazards which are reasonably likely to cause illness of humans are parasites (Trematodae, Nematodae, Cestodae), bacteria (Salmonella, E. coli, Vibrio parahemolyticus, Vibrio vulnificus, Listeria monocytogenes, Clostridium botulinum, Staphyloccocus aureus), viruses (Norwalk virus, Entero virusesi, Hepatitis A, Rotovirus) and bio-toxins. Upon completion of hazard analysis, any measure(s) that are used to control the hazard(s) should be described.

We describe two patients with diagnosis of chronic lymphocytic leukemia (CLL) in whom interphase fluo-rescence in situ hybridization (FISH) analysis revealed trisomy 12 and del(13)(q14.3) occurring in the same clone. These abnormalities are rarely seen together and the prognostic relevance of their coexistence is still unclear. According to some data, a probable adverse prognosis for this group of patients is suggested. Our patients have been in a stable phase of the disease for more than one year since the given abnormalities were documented in their karyotypes. Further study is necessary to determine the prognostic significance of coexistence of these abnormalities in CLL patients.

Ramonda serbica and Ramonda nathaliae, rare resurrection plants growing in the Balkan Peninsula, produce a high amount of phenolic compounds as a response to stress. The composition and size of bacterial communities in two rhizosphere soil samples of these plants were analyzed using a metagenomic approach. Fluorescent in situ hybridization (FISH) experiments together with DAPI staining showed that the metabolically active bacteria represent only a small fraction, approximately 5%, of total soil bacteria. Using universal bacteria - specific primers 16S rDNA genes were amplified directly from metagenomic DNAs and two libraries were constructed. The Restriction Fragment Length Polymorphism (RLFP) method was used in library screening. Amongst 192 clones, 35 unique operational taxonomic units (OTUs) were determined from the rhizosphere of R. nathaliae, and 13 OTUs out of 80 clones in total from the library of R. serbica. Representative clones from each OTU were sequenced. The majority of sequences from metagenomes showed very little similarity to any cultured bacteria. In conclusion, the bacterial communities in the studied soil samples showed quite poor diversity. .

Pathological lying is compulsive and impulsive, pervasive conduct of the individual, does not aim to achieve material gain, but sometimes there are also self-defeating quality. A small number of research works on intelligence or personality of people who exhibit pathological lying, because they are not operationalized criteria of disorder and methodology of the test assessment. The aim of this paper is presenting how psychological assessment can respond to a number of important questions: a differential diagnostic dilemma (distinguishing pathological lying of confabulations, delusions beliefs and simulation), what is the amount and structure of intelligence in these subjects, if the person proves neuropsychological deficits that are expected in the prefrontal localization, whether pathological lying beside respondent meets criteria for personality disorder, identifying roles / motives which lie in a dynamic personality, the family system and social environment. The paper outlines the specific psychological tests to be applied in order to respond to these questions and how to harmonize the methods of testing with patients who exhibit pathological lying.