Publisher: CSIC - Instituto de Ciencias del Mar (ICM)
Project: EC | ASSEMBLE Plus (730984)
The presence of phytoplankton parasites along the water column was explored at the Long Term Ecological Station MareChiara (LTER-MC) in the Gulf of Naples (Mediterranean Sea) in October 2019. Microscopy analyses showed diatoms dominating the phytoplankton community in the upper layers (0-20 m). Metabarcoding data from the water column showed the presence of Chytridiomycota predominantly in the upper layers coinciding with the vertical distribution of diatoms. Laboratory incubations of natural samples enriched with different diatom cultures confirmed parasitic interactions of some of those chytrids – including members of Kappamyces – with diatom taxa. The temporal dynamics of diatoms and chytrids was also explored in a three-year metabarcoding time-series (2011-2013) from surface waters of the study area and in sediment samples. Chytrids were recurrently present at low relative abundances, and some taxa found to infect diatoms in the incubation experiments were also identified in the ASV time-series. However, co-occurrence analyses did not show any clear or recurrent pairing patterns for chytrid and diatom taxa along the three years. The chytrid community in the sediments showed a clearly different species composition compared to the recorded in the water column samples, with higher diversity and relative abundance. The combination of observations, incubations and metabarcoding confirmed that parasites are a common component of marine protist communities at LTER-MC. Host-parasite interactions must be determined and quantified to understand their role and the impact they have on phytoplankton dynamics File1: VERDI_samples_parameters.xlsx - Physico-chemical variables obtained from CTD profile - Inorganic nutrients concentrations - Chlorophyll-a concentrations - Organic carbon and nitrogen concentrations - Phytoplankton abundances - Detections of chytrids File 2: VERDI_asv_table.tbl: ASV abundances from natural samples and incubations File 3: VERDI_tax_table.tbl: Taxonomic assignments of ASVs File 4: VERDI_asv_seqs.fa: Sequences of ASVs File 5: VERDI_incubations_images.zip - Compilation of images taken during incubations with diatoms - Physico-chemical variables obtained from CTD profile - Inorganic nutrients concentrations - Chlorophyll-a concentrations - Organic carbon and nitrogen concentrations - Phytoplankton abundances - Detections of chytrids - Metabarcoding ASV abundances from natural samples and incubations - Metabarcoding Taxonomic assignments of ASVs - Metabarcoding Sequences of ASVs - Compilation of images taken during incubations with diatoms - European Union’s Horizon 2020 research and innovation programme under grant agreement No 730984, ASSEMBLE Plus project. - Spanish MICINN Project SMART (PID2020-112978GB-I00) - The research program LTER-MC is funded by the Stazione Zoologica Anton Dohrn Peer reviewed
The database provides discrete measurements of carbon system parameters in water samples collected at 3 stations that form the marine time series GIFT during 33 oceanographic campaigns conducted over 2005–2021. Geographic coordinates of sampling stations are provided. Some physical data (i.e. pressure, temperature and salinity) are also included. Moreover, pH data obtained with a SAMI-pH sensor (Sunburst Sensors, LLC)) attached to a mooring line deployed in the Strait of Gibraltar for the years 2016 and 2017 are provided. During the cruises, a temperature and salinity profile was obtained with a Seabird 911Plus CTD probe. Seawater was subsequently collected for biogeochemical analysis using Niskin bottles immersed in an oceanographic rosette platform at variable depths (from 5 to 8 levels) depending on the instant position of the interface between the Atlantic and Mediterranean flows that was identified by CTD profiles. The biogeochemical variables shown in the database are pH in total scale at 25 °C (pHT25), total alkalinity (AT), and inorganic nutrients (phosphate, PO43and Silicate, SiO44−). pHT25 data were obtained by the spectrophotometric method with m-cresol purple as the indicator (Clayton & Byrne 1993). Samples were taken directly from the oceanographic bottles in 10 cm path-length optical glass cells and measurements were carried out with a Shimadzu UV-2401PC spectrophotometer containing a 25 °C-thermostated cells holder. Samples for AT analysis were collected in 500-ml borosilicate bottles, and poisoned with 100 μl of HgCl2-saturated aqueous solution and stored until measurement in the laboratory. AT was measured by potential titration according to Mintrop et al. (2000) with a Titroprocessor (model Metrohm 794 from 2005-2020 and model Metrothm 888 for 2021). Water samples (5 mL, two replicates) for inorganic nutrients determination were taken, filtered immediately (Whatman GF/F, 0.7 μm) and stored frozen for later analyses in the shore-based laboratory. Nutrients concentrations were measured with a continuous flow auto-analyzer using standard colorimetric techniques (Hansen & Koroleff 1999). 2. Methods for processing the data: 3. Instrument- or software-specific information needed to interpret/reproduce the data, please indicate their location: 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission, please specify using CREDIT roles https://casrai.org/credit/: Chief Scientists -I.Emma Huertas/Susana Flecha; Hydro: Who -Susana Flecha/David Roque/Silvia Amaya-Vías/Angélica Enrique; Nuts: Who -Manuel Arjonilla/ Status - final; Silicate and Phosphate Autoanalizer Hansen and Koroleff (1999) This research was supported by the COMFORT project that has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 820989 (project COMFORT, "Our common future ocean in the Earth system – quantifying coupled cycles of carbon, oxygen, and nutrients for determining and achieving safe operating spaces with respect to tipping points).” Funding was also provided by the European projects CARBOOCEAN (FP6-511176), CARBOCHANGE (FP7-264879), PERSEUS (FP7-287600) and the Junta de Andalucía TECADE project (PY20_00293). The dataset is subject to a Creative Commons License Attribution-ShareAlike 4.0 International. F.F.P. was supported by the BOCATS2 (PID2019-104279GB-C21) project funded by MCIN/AEI/10.13039/501100011033. SAV was supported by a pre-doctoral grant FPU19/04338 from the Spanish Ministry of Science, Innovation and Universities. Peer reviewed
Publisher: Institute of Marine Sciences (ICM-CSIC)
The marine Biological Reference Collections (CBR) are located at the Institute of Marine Sciences (ICM-CSIC) in Barcelona, Spain. The CBR are a Unit of Service where around 15000 referenced species are preserved, catalogued and maintained for their study. The most represented marine groups at the CBR are fish, crustaceans, molluscs and echinoderms, but also other groups are present. The studies based on the CBR specimens are focused on biodiversity, biogeography, taxonomy (type species), invasive and alien species, and genetic analysis. Several PhD theses have also been carried out in collaboration with the CBR.The CBR are a reference point for the marine biodiversity of the Mediterranean Sea, but in their facilities the CBR also hold specimens from all the oceans (Atlantic, Pacific, Indian, Antarctic and Arctic). The Collections are constantly receiving new specimens and updating. The main sources of specimens are oceanographic surveys and different kind of sampling programs carried out by the research projects run by the ICM-CSIC. However, the CBR have also received (in the past and currently) different collections donated by naturalists, researchers, other institutions, and particulars. The CBR were created in 1981, in the earlier history of the ICM-CSIC, by Jaume Rucabado, Domingo Lloris and Concepción Allué. The Collections were later recognized and catalogued by the Spanish Ministry of Culture in 1990. In the last decade, the CBR initiated a new stage where the information was digitized and the physical preservation of specimens updated to the new rules (such as change from formaldehyde to ethanol). The CBR are now part of GBIF (Global Biodiversity Information Facility), thus making public and available all data collections and their metadata. We have also incorporated the use of Geographical Information Systems (GIS) to monitor and study the geographical distribution of our specimens and moreover, the CBR started to act as repository of DNA voucher collections for genetic analyses.As a unit of service of the ICM-CSIC we think that education and outreach of marine science is of crucial importance for the society and for that reason the CBR take active part in several outreach activities with schools, universities and general public. For more information or details you can visit our webpage (http://cbr.icm.csic.es/en/node) and send us an e-mail (firstname.lastname@example.org). We will be happy to help you. Peer reviewed
Publisher: Institute of Marine Sciences (ICM-CSIC)
The occurence of white grouper is reported according to digital data mined on YouTube regarding recreational fishing of this species. The location of the catch has been extracted from the title and description of the video or from the video itself. Data associated to the following publication:https://www.sciencedirect.com/science/article/abs/pii/S004896972036201X
The Daily Egg Production Method (DEPM) to estimate the Anchovy Spawning Stock Biomass (SSB) in the Gulf of Cádiz (ICES, Subdivision 9a South) is conducted by Spain (Centro Nacional Instituto Español de Oceanografía, CSIC) every three years, since 2005. BOCADEVA 0720 is the sixth survey of the historical DEPM series for anchovy in the Gulf of Cádiz and was delivered on board R/V Ramón Margalef (CNIEO) from the 9th to the 17th of July 2020. The surveyed area extended from Strait of Gibraltar to Cape San Vicente (Spanish and Portuguese waters in the Gulf of Cadiz). Plankton samples, along a grid of 21 transects perpendicular to the coast were obtained for the spawning area delimitation and density estimation of the daily egg production. The survey objectives also included the characterization of the oceanographic and meteorological conditions in the study area. The samples to estimate adult parameters (sex ratio, female mean weight, batch fecundity and spawning fraction) were obtained in the acoustic survey “ECOCADIZ 2020-07”, carried out during the same period. This working document provides a d escription of the survey, laboratory analysis and estimation procedures used to obtain the Gulf of Cadiz Anchovy SSB by DEPM for 2020 in the South-Atlantic Iberian Stock.
Publisher: PANGAEA - Data Publisher for Earth & Environmental Science
Project: EC | TRIATLAS (817578)
The values of natural abundance of stable isotopes were measured in 13 micronekton fish species sampled during the MAFIA cruise (North Atlantic, April 2015). This dataset contains the values obtained for carbon and nitrogen in bulk tissues, and nitrogen values in amino acids. Length data and the number of individuals analysed for each species are also provided. Mesopelagic fishes were collected using a ''Mesopelagos” net (5x7 m mouth opening, 58 m total lenght) equipped with graded-mesh netting (starting with 30 mm and ending with 4 mm) and a multi-sampler for collecting samples from 5 different depth layers (Olivar et al., 2017). For C:N and stable isotope analyses, individual fish were eviscerated, freeze-dried and weighted. Aliquots of muscular tissue (or whole individuals for species of small size) were analyzed in an elemental analyzer (bulk tissues, Olivar et al., 2019) or a gas chromatograph (derivatized amino acids, Mompeán et al., 2016) coupled to isotope-ratio mass spectrometers. This research was funded by projects MAFIA (CTM2012-39587-C04), BATHYPELAGIC (CTM2016-78853-R), and QLOCKS (PID2020-115620RB-100) from the Plan Estatal de I+D+I (Spain), projects SUMMER (Grant Agreement 817806) and TRIATLAS (Grant Agreement 817578), from the European Union (Horizon 2020 Research and Innovation Programme), and the support through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S).