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Funded European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429) (PARAFISHCONTROL project) Assembled transcripts obtained from raw sequence reads after RNA-seq analysis of cultured P. dicentrarchi B1, C1 and I1 strains, obtained after RNAseq of cultured ciliates. Ciliates were isolated from experimentally infected turbot and cultured for three weeks in L-15 medium with 10% FCS at 18 oC. Then, ciliates were subjected to transcriptome analysis by using RNAseq

Funded by PARAFISHCONTROL project, European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 These files contain the annotated genes of P. dicentrarchi B1, I1 and C1 strains, obtained after RNAseq of cultured ciliates. Ciliates were isolated from experimentally infected turbot and cultured for three weeks in L-15 medium with 10% FCS at 18 oC. Then, ciliates were subjected to transcriptome analysis by using RNAseq

Settling particles were collected using sediment traps deployed along three transects in the Lacaze-Duthiers and Cap de Creus canyons and the adjacent southern open slope from October 2005 to October 2006. The settling material was analyzed to obtain total mass fluxes and main constituent contents (organic matter, opal, calcium carbonate, and siliciclastics). Cascades of dense shelf water from the continental shelf edge to the lower continental slope occurred from January to March 2006. They were traced through strong negative near-bottom temperature anomalies and increased current speeds, and generated two intense pulses of mass fluxes in January and March 2006. This oceanographic phenomenon appeared as the major physical forcing of settling particles at almost all stations, and caused both high seasonal variability in mass fluxes and important qualitative changes in settling material. Fluxes during the dense shelf water cascading (DSWC) event ranged from 90.1 g m−2 d−1 at the middle Cap de Creus canyon (1000 m) to 3.2 g m−2 d−1 at the canyon mouth (1900 m). Fractions of organic matter, opal and calcium carbonate components increased seaward, thus diminishing the siliciclastic fraction. Temporal variability of the major components was larger in the canyon mouth and open slope sites, due to the mixed impact of dense shelf water cascading processes and the pelagic biological production. Results indicate that the cascading event remobilized and homogenized large amounts of material down canyon and southwardly along the continental slope contributing to a better understanding of the off-shelf particle transport and the internal dynamics of DSWC events.

In June 2009, 22 spectrometers from 14 institutes measured tropospheric and stratospheric NO2 from the ground for more than 11 days during the Cabauw Intercomparison Campaign of Nitrogen Dioxide measuring Instruments (CINDI), at Cabauw, NL (51.97° N, 4.93° E). All visible instruments used a common wavelength range and set of cross sections for the spectral analysis. Most of the instruments were of the multi-axis design with analysis by differential spectroscopy software (MAX-DOAS), whose non-zenith slant columns were compared by examining slopes of their least-squares straight line fits to mean values of a selection of instruments, after taking 30-min averages. Zenith slant columns near twilight were compared by fits to interpolated values of a reference instrument, then normalised by the mean of the slopes of the best instruments. For visible MAX-DOAS instruments, the means of the fitted slopes for NO2 and O4 of all except one instrument were within 10% of unity at almost all non-zenith elevations, and most were within 5%. Values for UV MAX-DOAS instruments were almost as good, being 12% and 7%, respectively. For visible instruments at zenith near twilight, the means of the fitted slopes of all instruments were within 5% of unity. This level of agreement is as good as that of previous intercomparisons, despite the site not being ideal for zenith twilight measurements. It bodes well for the future of measurements of tropospheric NO2, as previous intercomparisons were only for zenith instruments focussing on stratospheric NO2, with their longer heritage.

Four strains of the coccolithophore Emiliania huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Coccolith morphology was analyzed based on scanning electron micrographs. Three of the four strains did not exhibit a change in morphology over the CO2 range tested. One strain (RCC1256) displayed an increase in the percentage of malformed coccoliths with increasing CO2 concentration. We conclude that the sensitivity of the coccolith-shaping machinery to carbonate chemistry changes is strain-specific. Although it has been shown before that carbonate chemistry related changes in growth- and calcification rate are strain-specific, there seems to be no consistent correlation between coccolith morphology and growth or calcification rate. We did not observe an increase in the percentage of incomplete coccoliths in RCC1256, indicating that the coccolith-shaping machinery per se is affected by acidification and not the signalling pathway that produces the stop-signal for coccolith growth. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-01-20. Supplement to: Langer, Gerald; Probert, Ian; Nehrke, Gernot; Ziveri, Patrizia (2011): The morphological response of Emiliania huxleyi to seawater carbonate chemistry changes: an inter-strain comparison. Journal of Nannoplankton Research, 32(1), 29-34

Since the start of the HERMES and the HERMIONE projects, the margin of the Bay of Biscay has received special attention with respect to benthic ecosystems and sedimentary processes. The area is also known to be the historical cradle of cold-water coral studies by Joubin (1922) and Le Danois (1948). Already at that time the relationship between cold-water corals and fisheries were being discussed (with corals being a nuisance for fisheries!). Having performed previous work in the Whittard canyon (R/V Belgica 2006) and the Guilvinec canyon (R/V Belgica 2008), a joint marine geology and biology cruise was organized by the Renard Centre of Marine Geology and the Section of Marine Biology from Ghent University from 7 to 28 June 2010. The first leg (7-16 June, Zeebrugge-La Rochelle) focused on the Guilvinec canyon, while the second leg (19-28 June, La Rochelle-Zeebrugge) revisited several sites in the central Whittard canyon. The main objectives of this cruise were to (a) map and observe cold-water coral ecosystems on the canyon flanks with ROV Genesis, (b) obtain hydrographic data from CTD casts and water samples and (c) perform seabed multicoring for biology (descriptive and experimental research), biogeochemistry and sedimentology. In total, 23 scientists participated in this cruise, representing 10 institutes, among which were IFREMER, University College Cork and IFM-GeoMAR. The HERMIONE project is funded by the European Commission's Framework 7 Programme, under the theme "Environment (including climate change)". EC contract no. 226354. Peer reviewed 11 páginas