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Folder with two datasets of enzyme activities (CS and LDH) with associated details of fish, incuding genotypes, body size, and metabolic rates, and R codes for linear mixed models as described in the manuscript Prokkola et al (submitted 2023). See README file for more information.

Key to taxa for Figure 5, figures S11A and S11B

Complementary figures and details about the article

The data is from a mesocosm experiment set up outside Lima, Peru to study the influence of upwelling of oxygen minimum zone (OMZ) water.The mesocosm bags were 2 m in diameter and extended from the surface down to 19 m depth, where the last 2 m was a conical sediment trap. Eight mesocosm bags were used and they were moored at 12.0555°S; 77.2348°W just north of Isla San Lorenzo where the water depth is ~30 m. The experiment was started 25 February 2017 by closing the mesocosm bags and were run for 50 days.Two treatments were used (water with different OMZ signature), each with four replicates. Water (100 m3) from the OMZ was collected from two locations and depths. The first was collected from 12.028323°S; 77.223603°W from 30 m depth, and the second one from 12.044333°S; 77.377583°W from 70 m depth. The original aim was to collect severe and moderate OMZ signature water (differing in e.g. nitrate concentrations) from the first and second site, respectively. This assumption was based on long-term monitoring data, however, the chemical properties (e.g. nitrate concentration) was more similar in these water masses than anticipated, rather reflecting low and very low OMZ signatures from site 1 and 2 respectively.To have a baseline of measured variables, the mesocosms where closed and environmental and biological variables were determined over 10 days. After this period, the OMZ water was added to the mesocosms in two steps on day 11 and 12 after the enclosure of the mesocosms. As the mesocosms contain a specific volume (~54 m3), the process of adding the OMZ water started with first removing water from the mesocosms. The water removed (~20 m3) was pumped out from 11-12 m depth. A similar volume of OMZ water, from both collection sites, was then pumped into four replicate mesocosms each. The OMZ water was pumped into the mesocosms moving the input hose between 14-17 m depth. The water collected at 30 m depth was pumped into mesocosms M1, M4, M5 and M8 having a low OMZ signature and water from 70 m depth into mesocosms M2, M3, M6 and M7 having a very low OMZ signature. Due a halocline at 12 m depth (see below), the added OMZ water was not immediately mixed throughout the mesocosm bag.Sampling took place every second day over a period of 50 days, and all variables were taken with an integrated water sampler (HydroBios, IWS) pre-programed to fill from 0 – 10 m depth and all samples consisted of this integrated samples from the upper 10 m. The samples were stored dark in cool boxes and brought back to the laboratory and processed right away. Sampling took place in the morning, and the samples were usually back in the laboratory around noon.Measured variables included inorganic nutrients, dissolved organic nutrients, extracellular enzyme activity: leucine aminopeptidase (LAP) and alkaline phosphatase activity (APA), and the phytoplankton and bacterial community composition. Bacteria are given in percent of total operational taxonomic unit.

The data is from a mesocosm experiment set up outside Lima, Peru to study the influence of upwelling of oxygen minimum zone (OMZ) water. The mesocosm bags were 2 m in diameter and extended from the surface down to 19 m depth, where the last 2 m was a conical sediment trap. Eight mesocosm bags were used and they were moored at 12.0555°S; 77.2348°W just north of Isla San Lorenzo where the water depth is ~30 m. The experiment was started 25 February 2017 by closing the mesocosm bags and were run for 50 days. Two treatments were used (water with different OMZ signature), each with four replicates. Water (100 m3) from the OMZ was collected from two locations and depths. The first was collected from 12.028323°S; 77.223603°W from 30 m depth, and the second one from 12.044333°S; 77.377583°W from 70 m depth. The original aim was to collect severe and moderate OMZ signature water (differing in e.g. nitrate concentrations) from the first and second site, respectively. This assumption was based on long-term monitoring data, however, the chemical properties (e.g. nitrate concentration) was more similar in these water masses than anticipated, rather reflecting low and very low OMZ signatures from site 1 and 2 respectively. To have a baseline of measured variables, the mesocosms where closed and environmental and biological variables were determined over 10 days. After this period, the OMZ water was added to the mesocosms in two steps on day 11 and 12 after the enclosure of the mesocosms. As the mesocosms contain a specific volume (~54 m3), the process of adding the OMZ water started with first removing water from the mesocosms. The water removed (~20 m3) was pumped out from 11-12 m depth. A similar volume of OMZ water, from both collection sites, was then pumped into four replicate mesocosms each. The OMZ water was pumped into the mesocosms moving the input hose between 14-17 m depth. The water collected at 30 m depth was pumped into mesocosms M1, M4, M5 and M8 having a low OMZ signature and water from 70 m depth into mesocosms M2, M3, M6 and M7 having a very low OMZ signature. Due a halocline at 12 m depth (see below), the added OMZ water was not immediately mixed throughout the mesocosm bag. Sampling took place every second day over a period of 50 days, and all variables were taken with an integrated water sampler (HydroBios, IWS) pre-programed to fill from 0 – 10 m depth and all samples consisted of this integrated samples from the upper 10 m. The samples were stored dark in cool boxes and brought back to the laboratory and processed right away. Sampling took place in the morning, and the samples were usually back in the laboratory around noon. Measured variables included inorganic nutrients, dissolved organic nutrients, extracellular enzyme activity: leucine aminopeptidase (LAP) and alkaline phosphatase, and the phytoplankton and bacterial community composition.

The data is from a mesocosm experiment set up outside Lima, Peru to study the influence of upwelling of oxygen minimum zone (OMZ) water. The mesocosm bags were 2 m in diameter and extended from the surface down to 19 m depth, where the last 2 m was a conical sediment trap. Eight mesocosm bags were used and they were moored at 12.0555°S; 77.2348°W just north of Isla San Lorenzo where the water depth is ~30 m. The experiment was started 25 February 2017 by closing the mesocosm bags and were run for 50 days. Two treatments were used (water with different OMZ signature), each with four replicates. Water (100 m3) from the OMZ was collected from two locations and depths. The first was collected from 12.028323°S; 77.223603°W from 30 m depth, and the second one from 12.044333°S; 77.377583°W from 70 m depth. The original aim was to collect severe and moderate OMZ signature water (differing in e.g. nitrate concentrations) from the first and second site, respectively. This assumption was based on long-term monitoring data, however, the chemical properties (e.g. nitrate concentration) was more similar in these water masses than anticipated, rather reflecting low and very low OMZ signatures from site 1 and 2 respectively. To have a baseline of measured variables, the mesocosms where closed and environmental and biological variables were determined over 10 days. After this period, the OMZ water was added to the mesocosms in two steps on day 11 and 12 after the enclosure of the mesocosms. As the mesocosms contain a specific volume (~54 m3), the process of adding the OMZ water started with first removing water from the mesocosms. The water removed (~20 m3) was pumped out from 11-12 m depth. A similar volume of OMZ water, from both collection sites, was then pumped into four replicate mesocosms each. The OMZ water was pumped into the mesocosms moving the input hose between 14-17 m depth. The water collected at 30 m depth was pumped into mesocosms M1, M4, M5 and M8 having a low OMZ signature and water from 70 m depth into mesocosms M2, M3, M6 and M7 having a very low OMZ signature. Due a halocline at 12 m depth (see below), the added OMZ water was not immediately mixed throughout the mesocosm bag. Sampling took place every second day over a period of 50 days, and all variables were taken with an integrated water sampler (HydroBios, IWS) pre-programed to fill from 0 – 10 m depth and all samples consisted of this integrated samples from the upper 10 m. The samples were stored dark in cool boxes and brought back to the laboratory and processed right away. Sampling took place in the morning, and the samples were usually back in the laboratory around noon. Measured variables included inorganic nutrients, dissolved organic nutrients, extracellular enzyme activity: leucine aminopeptidase (LAP) and alkaline phosphatase activity (APA), and the phytoplankton and bacterial community composition.

Auto-CO-AFM is an open-source software package for scanning probe microscopes that enables the automatic functionalization of scanning probe tips with carbon monoxide molecules. This enables machine operators to specify the quality of the tip needed utilizing a pre-trained library with off-the-shelf software. From a single image, the software package can determine which molecules on a surface are carbon monoxide, perform the necessary tip functionalization procedures, interface with microscope software to control the tip position, and determines the centeredness of the tip after a successful functionalization. This is of particular interest for atomic force microscopy imaging of molecules on surfaces, where the tip functionalization is a necessary and time consuming step needed for sub-molecular resolution imaging. This package is freely available under the MIT License. THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOVE

The tethered balloon system BELUGA (Balloon-bornE moduLar Utility for profilinG the lower Atmosphere) was operated during leg 4 of the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC). The balloon was operated from the Balloon Town site in the central observatory, close to RV Polarstern (Shupe et al., 2022, Elementa). Balloon payload included an extended meteorological package, an ultrasonic anemometer package, a broadband radiation package, the video ice particle sampler, and the cubic aerosol measurement platform. An overview showing the value of the combined observation is displayed by Lonardi et al. (in review). The data processing is described in Pilz et al. (in preparation). The present dataset covers the solar irradiances measured by the broadband radiation package on 18 flights between 29 June and 27 July 2020. Profiles of downward and upward raw solar radiation, latitude, longitude, roll, pitch, yaw, and radiometer icing flag. Geopotential height is derived from the pressure.

The tethered balloon system BELUGA (Balloon-bornE moduLar Utility for profilinG the lower Atmosphere) was operated during leg 4 of the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC). The balloon was operated from the Balloon Town site in the central observatory, close to RV Polarstern (Shupe et al., 2022, Elementa). Balloon payload included an extended meteorological package, an ultrasonic anemometer package, a broadband radiation package, the video ice particle sampler, and the cubic aerosol measurement platform. An overview showing the value of the combined observation is displayed by Lonardi et al. (in review). The data processing is described in Pilz et al. (in preparation). The present dataset covers the solar irradiances measured by the broadband radiation package on 18 flights between 29 June and 27 July 2020. Profiles of downward and upward raw solar radiation, latitude, longitude, roll, pitch, yaw, and radiometer icing flag. Geopotential height is derived from the pressure.