Starting from a unique collection of paleo-environmental samples (frozen Arctic soils and sediments) already at AWI and their corresponding ancient DNA (aDNA) metagenomes that stretch back up to a million years, we will retrieve information on how rhizosphere biodiversity and functionality responded to climate changes and extreme events. Preliminary metagenomic data from the collection suggests it is a gold mine of archaic DNA that represents a timeline of adaptions to climate change in the rhizosphere. By reconstructing and analyzing these ancient metagenomes and correlating with available historical climatic change data, we will identify molecular adaptions that impart climate tolerance (specifically resistance to increased temperature and drought). This will be used to i) produce and test engineered root-colonizing bacteria (Pseudomonas fluorescens and Pseudomonas chlororaphis) that will improve climate tolerance of plants (production of humidifying polysaccharides around the root) facilitating the ability to grow on marginal agricultural land (MAL), ii) inform ancestral reconstruction of thermostable and/or cold tolerant enzymes for industrial application and iii) produce engineered Pseudomonas putida strains tailored for bioproduction. For the latter application, we will select genes that encode biomolecules relevant for climate-tolerant phenotypes (humidifying polysaccharides and the biosurfactant betaines). The production of these molecules using biotechnology will be targeted in the project and their application in selected industrial products verified. Target end-user applications will include polysaccharides and betaines for the development of 3D printed organ-on-chip and drug delivery systems as well as the formulation of metalworking fluids, lubricants and industrial cleaning products.

CITRUSBUSTERS proposes an ambitious and efficient R&I initiative aimed at developing solutions tailored to address Citrus pests with devastating impacts worldwide: Candidatus Liberibacter (CL), its associated insect vector (T. erytreae) and Phyllostycta citricarpa (PC), considered priority and quarantine pest in the EU. With an approach prioritising prevention, CITRUSBUSTERS will enlarge the IPM toolbox for high-value citrus crops by: (i) demonstrating the feasibility and benefits of smart tools for pathogen observation and early detection, enabling the prediction of future outbreak risks, based on co-culture models for CL and PC to provide solid knowledge on their spatiotemporal dynamics, technologies based on hyperspectral and LIDAR imaging, and predictive models through MLA linking soil health, vectors and plant parameters; ii) developing innovative tools, substances and agroecological practices to enhance plant resilience against biotic stresses by means of DNA-free and genetic changes for breeding purposes and innovative extraction technologies for active molecules triggering immune responses in plants; and (iii) developing sustainable and safe biocontrol active formulations for improving crop health during cultivation, by means of encapsulated endolysins for CL, microbial antifungal substances for PC and dsRNA targeting T.erytreae. CITRUSBUSTERS will strenghten the abilities of stakeholders to respond to pest outbreaks relying on a solid multiactor approach for the co-creation of solutions and the assessment of environmental, social and economic impacts of the pests of the solutions, developing policy recommendations and best practices guidelines. This will ensure the adequacy of the proposed solutions and the wider acceptance of IPM strategies contributing to promote fair, healthy and environment-friendly food systems from primary production to consumption and to restoring Europe’s ecosystems and biodiversity.

Roughly 30% of Nationally Determined Contributions (NDCs) under the Paris Agreement (PA) include land-based mitigation measures, but there are still significant uncertainties in their effectiveness to deliver negative emissions. Aside from the expected shortfall of all current NDCs to deliver on the below 2 oC ambition, this uncertainty adds to the risks to human wellbeing as a result of climate change. Land-use based mitigation technologies (LMTs) can play a crucial role in the global efforts to meet the PA goals and the Sustainable Development Goals (SDGs). Considering the land-climate-development interface, LANDMARC aims to assess the impacts of LMTs as net sinks for greenhouse gas (GHGs) by applying unique mixed-methods approach. LANDMARC assesses the potential and feasibility of LMTs in the AFOLU sector by: a) quantitatively assessing environmental, social-economic, co-benefits and trade-offs identified through a suite of monitoring tools and model system (including land use, climate and economic models) complemented by; b) qualitative assessments guided by stakeholder engagement. This mixed-method approach allows us to provide more detailed insights on the effectiveness and climate resilience of LMTs at different spatial scales (e.g. scaling up from local/national level to the regional/global level). These tools, services and approaches will contribute to land-based LMT decision support in the private sector and by policy makers. LANDMARC is an interdisciplinary consortium with expertise from ecology, engineering, climate sciences, global carbon cycle, soil sciences, satellite earth observation sciences, agronomy, economics, social sciences, and business. There is a balanced representation of partners from academia, SMEs, and NGOs from the EU, Africa, Asia and the Americas, which ensures a wide coverage of LMTs operating in different contexts (e.g. climates, land-use practices, socio-economic etc.) and spatial scales.

Anaerobic digestion (AD) of organic matter is a robust technology for biogas synthesis from different types of waste (sewage sludge from water treatment, animal slurry, bio-waste, etc.). The main goal of AD is the production of methane, a renewable energy source that can be used to generate electricity, heat or as vehicle fuel. Biogas is a mixture of methane (CH4; 55–70% of the total volume), carbon dioxide (CO2; 30–40%) and traces of other gases. In 2018, EU was the world’s largest producer of biomethane, reaching 2,28 bcm. However, from a purely engineering view, the microbial process underlying methane production is considered to be a black box: it is subjected to a degree of variability and it is an industrial process with a lot of room for improvement in the systematic optimisation of (1) yield, (2) quality, (3) speed and (4) robustness of the process. MICRO4BIOGAS aims to tackle these 4 aspects by integrating, for the first time, the use of microbial consortia that naturally inhabit anaerobic digesters with synthetic microbial consortia with improved capabilities, setting the basis for a user-friendly kit for bioaugmentation of biogas production (activities will be implemented at TRL3 with a TRL target of 5-6). Partners from 6 EU countries will work side by side to make a difference in the European biogas industry, that individually could not be achieved. By improving the biogas production in Europe, this project meets the EU Bioeconomy Strategy and the European Green Deal, helping to reach the Sustainable Development Goals (SDG7: Affordable and clean energy; SDG13: Climate Action) and working towards the circularity, resource efficiency and sustainability of the European countries.