The duplication of genes provides new genetic material that can be used for novel functions, allowing plants and animals to evolve biological innovations and adapt to environmental conditions. Whole genome duplication (WGD) is arguably the most dramatic mechanism for duplication, resulting in the production of a new copy of every gene in the nuclear genome. Around 430 million years ago, spiders and scorpions diverged from a common ancestor that had experienced a WGD. The retained duplicated genes from this WGD event (genes called ohnologs) can still be found in the genomes of the approximately 45,000 species of these animals alive today and may have contributed to their adaptation and diversification. Since then, some families of Synspermiata spiders have undergone at least two additional WGDs within a single lineage, reflecting a similar series of WGDs in vertebrates. This presents an opportunity to compare these events to determine whether there are general principals shaping the outcomes of WGDs and their contribution to animal diversification. In addition, Synspermiata represent a wide diversity of spiders that are understudied and poorly understood Therefore, the aims of this project are to identify spider ohnologs after multiple WGDs, explore whether and how they have contributed to the evolutionary success of these animals, and compare the outcomes of these events to repeated WGDs in vertebrates. We will first collect and carry out the first large scale detailed study of the morphology of Synspermiata spiders to better understand their evolution and phenotypic diversity. In parallel, we will identify the ohnologs that have been retained in spider groups after WGDs by comparing the repertoire and arrangement of the duplicated genes in these animals with relatives where there is no evidence of additional WGDs. As part of this aim, we will sequence the genomes of Synspermiata spiders that have undergone one (Pholcus phalangioides, Scytodes thoracica and Loxosceles reclusa), and two (Oonops pulcher, Segestria senoculata and Dysdera crocata) WGD, as well as the transcriptomes of Caponiidae species with two (Orthonops zebra) or three (Calponia harrisonfordi) WGDs. Since relatively little is known about these spiders this will provide new insights into the biology of these animals as well as their genome evolution. We will then compare the repertoires of genes retained after WGD between spiders and vertebrates to determine whether there are any similarities in the aftermath of these events. This information will help us to better understand the general consequences of WGD and the principles underlying their outcomes in terms of genes being preferentially retained or lost again. Identification of ohnologs will also allow us to ask if these genes have been subject to sub-, neofunctionalisation or specialisation during spider development and if their expression is associated with morphological diversification. Overall our project will provide new insights into the genomes of spiders and how WGDs in these animals have contributed to their morphological evolution. Our data will also allow comparisons to WGD events in other animals, including vertebrates, to better understand the general consequences of these events and their contribution to animal adaptation and diversification.

The duplication of genes provides new genetic material that can be used for novel functions, allowing plants and animals to evolve biological innovations and adapt to environmental conditions. Whole genome duplication (WGD) is arguably the most dramatic mechanism for duplication, resulting in the production of a new copy of every gene in the nuclear genome. Around 430 million years ago, spiders and scorpions diverged from a common ancestor that had experienced a WGD. The retained duplicated genes from this WGD event (genes called ohnologs) can still be found in the genomes of the approximately 45,000 species of these animals alive today and may have contributed to their adaptation and diversification. Since then, some families of Synspermiata spiders have undergone at least two additional WGDs within a single lineage, reflecting a similar series of WGDs in vertebrates. This presents an opportunity to compare these events to determine whether there are general principals shaping the outcomes of WGDs and their contribution to animal diversification. In addition, Synspermiata represent a wide diversity of spiders that are understudied and poorly understood Therefore, the aims of this project are to identify spider ohnologs after multiple WGDs, explore whether and how they have contributed to the evolutionary success of these animals, and compare the outcomes of these events to repeated WGDs in vertebrates. We will first collect and carry out the first large scale detailed study of the morphology of Synspermiata spiders to better understand their evolution and phenotypic diversity. In parallel, we will identify the ohnologs that have been retained in spider groups after WGDs by comparing the repertoire and arrangement of the duplicated genes in these animals with relatives where there is no evidence of additional WGDs. As part of this aim, we will sequence the genomes of Synspermiata spiders that have undergone one (Pholcus phalangioides, Scytodes thoracica and Loxosceles reclusa), and two (Oonops pulcher, Segestria senoculata and Dysdera crocata) WGD, as well as the transcriptomes of Caponiidae species with two (Orthonops zebra) or three (Calponia harrisonfordi) WGDs. Since relatively little is known about these spiders this will provide new insights into the biology of these animals as well as their genome evolution. We will then compare the repertoires of genes retained after WGD between spiders and vertebrates to determine whether there are any similarities in the aftermath of these events. This information will help us to better understand the general consequences of WGD and the principles underlying their outcomes in terms of genes being preferentially retained or lost again. Identification of ohnologs will also allow us to ask if these genes have been subject to sub-, neofunctionalisation or specialisation during spider development and if their expression is associated with morphological diversification. Overall our project will provide new insights into the genomes of spiders and how WGDs in these animals have contributed to their morphological evolution. Our data will also allow comparisons to WGD events in other animals, including vertebrates, to better understand the general consequences of these events and their contribution to animal adaptation and diversification.

Counting animals - and the number of habitats occupied by animals - is fundamental to conservation decision-making. Despite recent advances in survey design and analysis, population assessments of amphibians and reptiles almost entirely rely on simple counts that usually bear little relationship to actual population sizes, densities or the number of habitats occupied. This is because simple counts fail to take into account variations in the detectability of animals between habitats, time periods or observers. Consequently, the quality of data collected on amphibian and reptile populations is extremely variable. We have been developing and testing survey methods for assessing the population status of a variety of amphibian and reptile species, that use designs and analytical tools that take account of variations in detectability. Because of the recent growth in interest in assessing the status of protected amphibians and reptiles, we believe that the time is right to make such tools more widely available to professional end-users. We therefore seek funds from NERC to make this happen, and put amphibian and reptile population assessment on a par with other taxa that have well-defined survey standards. The stakeholders involved with the project will be statutory agencies that provide advice and licensing for protected species; local authorities responsible for planning decisions and managing local nature reserves; ecological consultants who carry out surveys and mitigation projects on behalf of developers; and conservation organisations that provide advice, training and site management on amphibian and reptile issues. The project will be divided into three phases which will be overseen by a steering group that includes principal stakeholders. Phase I will comprise a series of regional workshops that will: (1) brief regional end-users on recent developments in tools to design and analyse survey data, available software, and how these might be applied to amphibian and reptile data; and (2) seek feedback on what the current pressing issues are in population assessments, the range of methods used and their advantages and disadvantages, and potential obstacles to applying new methodologies and analyses. From these workshops we will draft new standard protocols for survey design and analysis. In Phase II of the project the draft protocols will be 'road-tested' on real survey projects being carried out by end-users. We will provide a design and analysis service for a number of such projects, and in return the end-users will provide feedback on the performance of the draft protocols. In this way, end-users will gain hands-on experience of using statistical models and new protocols. In Phase III of the project, feedback from the road-tests will lead to revised, final survey protocols that will then be disseminated via the project partner network. In addition, we will run a training workshop that will ensure that more tightly defined best practice guidelines are adopted. We therefore intend the whole process to be one of knowledge exchange - rather than just knowledge transfer. The economic impact of the project will be widespread. With several million pounds being spent on development mitigation for amphibians and reptiles, debate rages over the cost-effectiveness of such actions. The protocols that will emerge from this project will therefore allow ecological consultants to produce much more effective mitigation plans for their clients. Equally, it will strengthen the decision-making processes involved with licensing by the statutory agencies, and local authorities will be better-informed when it comes to planning issues. Managers of nature reserves will also be able to carry out more effective surveys of amphibians and reptiles which will enhance our knowledge of the regional and national status of these animals.

YORKSHIRE DALES ENVIRONMENT NETWORK INTRODUCTION In the Yorkshire Dales the long interaction of people and nature has created a place with a unique mix of natural beauty, wildlife and cultural heritage: giving us the habitats for which the Dales are recognised and the ecosystem services which they provide. Conserving and enhancing this rich environment involves reconciling economic, conservation, social and cultural requirements, each represented by different stakeholders. Protecting these special qualities and reconciling the diverse interests thus requires effective collaboration between many stakeholders. MISSION Our aim is the formal creation of a network of organisations involved in the care and management of the Yorkshire Dales. The network's mission is, through changes in philosophy and practice, to achieve a step improvement in the strength and breadth of communication and collaboration between its members. This will lead to the most effective use of the diverse range of skills and resources available for the benefit of the Dales landscape and people. BACKGROUND The formation of the network fits with the now overwhelming recognition that conservation must be practised at the landscape scale and that environmental protection will only be made more effective through better communication and collaboration between public, private and voluntary sectors, and government and local communities. The Dales are a microcosm of the national state, and a coherent geographical and administrative region in which we can address these concerns. Formation of the network will allow partners to benefit from the latest thinking, methods and findings direct from the research community and to benefit more effectively from each other's expertise and resources. The researchers will gain access to field sites and environmental data and with the partners be able to formulate research programmes of direct relevance to the community. By acting together, the network can address environmental and biodiversity management at all levels from the site-specific to the landscape scale. OBJECTIVES In order to formalise and make best use of the Network we will: i. TRAIN A COORDINATOR who will identify the partners' requirements and respond by - RUNNING A SERIES OF EVENTS that facilitate and enhance the planning and execution of the partners' own missions. Events include; ii. WORKSHOPS will focus primarily on the current and developing needs of the partners but promoting new initiatives. A primary aim of most workshops will be the formation of a working group of partners best equipped to address particular issues. WORKING GROUPS arising from the workshops will be tasked with the practical implementation of actions arising from the workshops iii. ANNUAL CONFERENCE will bring together partners to showcase the year's activities and to look to future projects and strategic development of the Network. It will bring together a wider range of partners than form the workshops and working groups, encouraging broader exchange of knowledge, expertise and collaboration. It will also be outward facing, with activities to engage public and press. iv. EXHIBITIONS to promote the activities of the Network and encourage participation beyond the Network v. WEBSITE development to build a lasting point of contact, information exchange and public interface TOGETHER WE WILL: > increase the breadth and depth of collaboration among network partners > promote a lasting change in culture and practice in the network