DyeAnotherWay aims at providing Doctoral Candidates with the skills necessary to lead the textile and food industries into a greener future by developing new sustainable dyeing methods exploiting bacterial genetic resources in an innovation-oriented way using cutting edge technology. The co-operation between the professional textile industry, commercial suppliers of analytical and biotechnological services, and scientists from nine Universities from ten countries addresses urgent needs in the non-academic sector and scientific hotspots in microbiology, bioeconomy and the textile and food industry simultaneously. Bacteria-based pigments, are the common research platform in combination with a broad spectrum of disciplines ranging from microbiology, chemistry, biotechnology, biochemistry, molecular biology, and bioinformatics to textile processing and market research. This will, in the near future, make a range of fully characterised biogenic dyes commercially available to the industry for use in dyeing both conventional and new textiles, allowing the industry to wean itself off petrochemical dyes in an economically viable way. The dyes will potentially find application beyond the textile and food industry, thus allowing many fields to benefit from their low carbon footprint production and their compatibility with human and environmental health. The 12 Doctoral Candidates will obtain multi- sectoral training in the relevant disciplines. In addition, training in research management, communication/presentation, creativity and entrepreneurship will provide key transferable skills for public and private sector employment, thereby maximizing the impact on the research and training on the fellows’ careers. The diversity of dissemination and communication activities guarantees the DCs' exposure to stakeholders in every sector and will help shape the future of education in this field.

SHEALTHY proposal aims to assess and develop an optimal combination of non-thermal sanitization, preservation and stabilization methods to improve the safety (inactivation of pathogens and spoilage microorganisms), while preserving the nutritional quality (up to 30%) and prolonging the shelf-life (up to 50%) of minimally processed F&V products. By combining and modulating non-thermal technologies with minimally processing operation, we will respond to consumers’ demand for fresh, healthy, convenient, sustainable and locally produced and additive-free food. The combined and optimised mild technologies will be demonstrated and validated in 2 business cases: Minimally processed fruits and vegetables and Fruit and vegetable-based juices & smoothies. Sanitization during washing will be optimized by applying in combination ultrasound (US), electrolysed water, plasma activated water, High Intensity Pulsed Light and Blue Light. Bioactive coating, active and intelligent packaging will be applied for quality preservation and shelf life extension of minimally processed F&V. F&V-based juices & smoothies will be stabilized by US and high pressure processing. For F&V by-product valorisation, US, pulsed electric field and membrane filtration will be used to extract bioactive compounds. Sustainable and flexible processing methods will be transferred and adapted to the need of local F&V micro and SMEs, interconnecting primary producers through novel cooperative business models and new logistics systems, to enhance the traceability and authenticity of raw materials along the F&V value chain. Commercial feasibility will be assessed, including consumer acceptance and regulatory, safety and environmental aspects. SHEALTHY will combine the technology trends and consumer needs to afford the business models, technology transfer and market orientation that will facilitate the transition towards a new collaborative agrifood ecosystem for traditional, local and rural SMEs around EU.

The main objective of BIOCON-CO2 is to develop and validate in industrially relevant environment a flexible platform to biologically transform CO2 into added-value chemicals and plastics. The versatility and flexibility of the platform, based on 3 main stages (CO2 solubilization, bioprocess and downstream) will be proved by developing several technologies and strategies for each stage that will be combined as puzzle pieces. BIOCON-CO2 will develop 4 MCFs based on low-energy biotechnological processes using CO2 from iron&steel industry as a direct feedstock to produce 4 commodities with application in chemicals and plastics sectors using 3 different biological systems: anaerobic microorganisms (C3-C6 alcohols by Clostridia), aerobic microorganisms (3-hydroxypropionic acid by Cupriavidus necator) and enzymes (formic acid by recombinant resting E. coli cells and lactic acid by multi-enzymatic system). The technologic, socio-economic and environmental feasibility of the processes will be assessed to ensure their future industrial implementation, replicability and transfer to other CO2 sources, such as gas streams from cement and electricity generation industries. BIOCON-CO2 will overcome the current challenges of the industrial scale implementation of the biotechnologies routes for CO2 reuse by developing engineered enzymes, immobilization in nanomaterials, genetic and metabolic approaches, engineered carbonic anhydrases, pressurized fermentation, trickle bed reactor using advanced materials and electrofermentation. The project aims to capture at least 4% of the total market share at medium term (1.4Mtonnes CO2/year) and 10% at long term (3.5Mtonnes CO2/year) contributing to reduce EU dependency from fuel oils and support the EU leadership in CO2 reuse technologies. Policy recommendations and public perception and acceptance will be explored and a commercialization strategy will be executed by a detailed exploitation plan and technology transfer.

The overall aim of ShapingBio is to support and accelerate bioeconomy innovation and the deployment of new knowledge in the EU and its member states. ShapingBio aims to provide evidence-based and concrete information and recommendations for better policy alignment and stakeholder actions to realize the cross-sectoral potential of the bioeconomy and to reduce the fragmentation across bio-based sectors and food system and policies across regions, domains and governance levels. These actions contribute significantly to the bioeconomy strategy and Action Plan, the farm to fork strategy, the EU Green Deal policy priorities and the EU's Climate ambition for 2030 and 2050. For that purpose, ShapingBio aims to create a better understanding and information basis of the bioeconomy innovation eco-system by providing a comprehensive mapping and analysis of initiatives, structures, policy instruments and key gaps related to the topics policy and governance, applied R&D and technology transfer, (cross-sectoral) collaboration and financing across the EU macro-regions and different sectors. This will be carried out in close collaboration with various types of stakeholders from different sectors, by ensuring the involvement of all the relevant actors. Multi-actor groups will be set up to support policy advice design, but also to propose new forms of matchmaking, networking and knowledge-transfer. Those propositions will be implemented and tested in around twenty events. Based on these actions, recommendations for an effective governance in terms of policy instrument and their mixes will be derived as well as guidelines for different stakeholder groups for better collaboration and exploitation of bioeconomy innovations. Follow-up events will be conducted, to ensure that the recommendations are understood, actively reflected and implemented into stakeholders' activities and action plans to foster the deployment of bio-based innovations.

This project aims to develop new processes and solve bottlenecks in the fermentative production of biosurfactants and specialty carbohydrates. Specifically, the project targets the development of innovative fermentation processes to produce the following compounds: 1. Glycolipid biosurfactants. The project targets four distinct classes of biosurfactants, specifically rhamnolipids, sophorolipids, xylolipids and mannosylerythritol-lipids with a wide range of application fields. 2. Specialty carbohydrates. Specifically, the project targets sialylated oligosaccharides, a class of very complex Human Milk Oligosaccharides that find application as a neutraceutical, pharmaceutical and cosmetic ingredient. For both product lines, microbial producer strains will be developed through metabolic engineering. The fermentation process and down-stream processing will be developed and optimized in order to obtain an industrial process. Second generation technology based on lignocellulosic substrates will also be developed. Sufficient amounts of the new products will be produced for application testing, in order to evaluate their market potential in a wide range of application fields. The technical, economic, environmental and social sustainability of the process over the whole value chain from biomass to product application will also be assessed, with an emphasis on identifying and addressing the bottlenecks in the innovation chain. A valorisation plan will be drafted to complete the innovation process. The project consortium has all the required players to obtain the expected impact: RTO's to address the research challenges in this project, an open innovation pilot plant to optimize and scale up the new processes, three biotech SMEs and three large industries to ensure the exploitation of the project results. In addition, two user groups (one for each product line) consisting of end-user companies are involved in the project.