Counting animals - and the number of habitats occupied by animals - is fundamental to conservation decision-making. Despite recent advances in survey design and analysis, population assessments of amphibians and reptiles almost entirely rely on simple counts that usually bear little relationship to actual population sizes, densities or the number of habitats occupied. This is because simple counts fail to take into account variations in the detectability of animals between habitats, time periods or observers. Consequently, the quality of data collected on amphibian and reptile populations is extremely variable. We have been developing and testing survey methods for assessing the population status of a variety of amphibian and reptile species, that use designs and analytical tools that take account of variations in detectability. Because of the recent growth in interest in assessing the status of protected amphibians and reptiles, we believe that the time is right to make such tools more widely available to professional end-users. We therefore seek funds from NERC to make this happen, and put amphibian and reptile population assessment on a par with other taxa that have well-defined survey standards. The stakeholders involved with the project will be statutory agencies that provide advice and licensing for protected species; local authorities responsible for planning decisions and managing local nature reserves; ecological consultants who carry out surveys and mitigation projects on behalf of developers; and conservation organisations that provide advice, training and site management on amphibian and reptile issues. The project will be divided into three phases which will be overseen by a steering group that includes principal stakeholders. Phase I will comprise a series of regional workshops that will: (1) brief regional end-users on recent developments in tools to design and analyse survey data, available software, and how these might be applied to amphibian and reptile data; and (2) seek feedback on what the current pressing issues are in population assessments, the range of methods used and their advantages and disadvantages, and potential obstacles to applying new methodologies and analyses. From these workshops we will draft new standard protocols for survey design and analysis. In Phase II of the project the draft protocols will be 'road-tested' on real survey projects being carried out by end-users. We will provide a design and analysis service for a number of such projects, and in return the end-users will provide feedback on the performance of the draft protocols. In this way, end-users will gain hands-on experience of using statistical models and new protocols. In Phase III of the project, feedback from the road-tests will lead to revised, final survey protocols that will then be disseminated via the project partner network. In addition, we will run a training workshop that will ensure that more tightly defined best practice guidelines are adopted. We therefore intend the whole process to be one of knowledge exchange - rather than just knowledge transfer. The economic impact of the project will be widespread. With several million pounds being spent on development mitigation for amphibians and reptiles, debate rages over the cost-effectiveness of such actions. The protocols that will emerge from this project will therefore allow ecological consultants to produce much more effective mitigation plans for their clients. Equally, it will strengthen the decision-making processes involved with licensing by the statutory agencies, and local authorities will be better-informed when it comes to planning issues. Managers of nature reserves will also be able to carry out more effective surveys of amphibians and reptiles which will enhance our knowledge of the regional and national status of these animals.

The Asian Hornet (Vespa velutina nigrithorax) [AH] is an invasive alien species that is rapidly spreading through Europe. It is an important emerging threat to wild and managed pollinators, as it is a voracious predator of insects. Over 50% of its diet has been reported to consist of honeybees, wild bees and wasps. The AH live in large nests producing 5,000-20,000 individuals and the workers hawk for prey at bees' nest entrances, and flower patches, picking off foragers and shutting down foraging activity to the detriment of the colony. AH have caused significant damage to honeybee colonies in France and Italy with increased mortality of 5-80% where AH has established, threatening the viability of beekeeping and honey production. Of additional and immediate concern is the damage it could cause to wild bee and wasp populations, and the pollination services they provide to crops and wild flowers, but these effects have not been quantified. An AH nest was first found in UK in September 2016, another nest found in 2017, and a third found in September 2018, signalling that establishment in the UK is imminent. The only means of halting the spread, or limiting it, is to find and destroy nests as soon as possible. There is thus an urgent need to develop a method to locate nests efficiently for management of this pest. In a ground-breaking pilot project (funded by Defra) we have radio-tracked individual AH flying back to their (previously un-discovered) nests. Our first aim is to test the range of use of radiotelemetry, and combine it with a new acoustic monitor giving early warning of AH attacks at bee hives. We will design a protocol using these technologies to reduce the speed of AH invasion (Objective 1). Our second aim is to measure the potential impact of AH as a pest of managed and wild pollinators in three ways: measuring the impact of AH predating at bee colonies (Objective 2); measure the proportions of different pollinator species in the AH diet using DNA sequencing (Objective 3) and measure whether AH disrupt pollinator networks at flower patches (Objective 4). Work will be carried out in France and the Channel Islands which are at different stages of the invasion timeline. Data on AH foraging and predation rates will be used to forecast the likely impact of these predators on managed and wild pollinators in the UK, and estimate impacts on crop pollination (Objective 5) using our models of bee colony and population dynamics, BEEHAVE and bumbleBEEHAVE. It is essential that this research takes place as soon as possible: the best opportunity to slow a UK invasion is during the next few years. EU governments are looking for methods to slow the spread of AH, and beekeepers strongly advocate the need for investment in this work, (see Supporting Letters from Defra, DoE Jersey, and beekeepers). The ~250,000 honeybee hives in the UK are estimated to be worth £150 mill to the economy in honey and pollination provision. The AH could trigger losses of £30-45 mill per year, and our technology to detect nests early could reduce AH economic impact by at least £10-15 mill per year. This will be achieved by sharing our protocol and evidence with policy makers: to help manage the invasion and increase knowledge and skills amongst beekeepers, agencies and pest controllers. These actions, together with raised public awareness, will boost chances of early nest detection at the AH invasion front. The reduction in environmental impact will also be significant, because wild bees are under threat already, so that a further reduction in these communities is likely to affect their vitally important ecological function as pollinators. The assembled team of researchers at Univ. of Exeter (Osborne, Tyler, Kennedy & Chaput) and partners (French researchers, Defra, Dept of Environment Jersey, the BBKA & Jersey beekeepers) have experience in animal tracking, pollinator science, molecular ecology and practical AH management to succeed in these goals.