Along the food chain, most wet surfaces are covered by biological pellicles called biofilms. Biofilms are composed of spatially organized microorganisms (possibly including pathogens) embedded in an extracellular polymeric matrix. This organic cement can act as a “protective shield” against the action of antimicrobials, thus raising serious problems of pathogens persistence and extra-use of chemical biocides in industrial settings. This is a hot topic for industrialist at a time in which contradictory influences operate: consumers are urgently demanding for safe food (free of bacterial pathogens and toxins), while the European regulation is changing and will likely prohibit some disinfectants in the next few years (Reach, EU directive biocide 98/8/EC). In this context, we recently discovered that planktonic bacilli propelled by flagella are able to tunnel deep within biofilms structures. These bacterial stealth swimmers create transient pores that increase macromolecular transfer within the biofilm. We hypothesized and proved that irrigation of the biofilm by swimmer bacteria can exacerbate killing of biofilm bacteria by facilitating penetration and action of disinfectants from the environment. The objective of this “proof of concept” proposal is to go one step further in the applicability of this new concept by selecting synergetic cocktails of hyper-motile bacteria that can increase biocide efficacy against unwanted biofilms on industrial surfaces. The direct societal and environmental implication of such biological strategy is the drastic reduction of chemical use for the control of pathogens in industrial settings. The long term objective is to entirely replace chemical biocides by natural and specific antimicrobials produced and delivered by bacterial stealth swimmers. The development of this “environmental friendly” approach necessitates new tools to analyze non-invasively such bacterial tunneling dynamics in a biofilm matrix. To address this scientific node, we have assembled in this project a multidisciplinary consortium involving microbiologists, microscopists, computer scientists, together with an industrial partner involved in microbial biotechnology.