The present ANR project aims at fabricating and exploring the properties of a special class of capsules called “ethosomes”, resulting from the self-assembly of phospholipids in the presence of water and ethanol. The main objective of the project is to develop the underpinning science required to address challenges in designing ethosomes based on phospholipids rich in n-3 Poly-Unsaturated Fatty Acids (PUFA). Ethosomes can be used in nutrition applications because of the substantial benefits of PUFA in human health. Moreover, due to the presence of ethanol, ethosomes present membrane fluidity properties that make them interesting delivery systems by the topical route. So far, the phospholipids used to fabricate conventional liposomes or ethosomes are extracted from soya or egg yolk sources. The food industry is generating large amounts of by-products, most of them remaining under valorised. In this project, we propose to produce phospholipids either from animal marine or vegetable sources using supercritical CO2 as a “green” extraction solvent. Ethanol will be mixed with CO2 in order to improve the extraction yield. Delivering phospholipids rich in n-3 PUFA is really challenging due to their susceptibility to chemical degradation. Indeed, polyunsaturated lipids are sensitive to heat, light and oxygen exposure. This causes product deterioration in terms of aroma, texture, shelf life and colour. Within this project, we will examine the impact of ethanol on the physical and chemical stability of liposomes containing a large fraction of n-3 PUFA chains under well-defined storage conditions. In addition to the high-resolution techniques already available, we will develop a novel technique to follow lipid oxidation based on micro-calorimetry. Indeed, non-destructive, simple and efficient technique to follow the complex oxidation process is highly sought-after and not presently available.