Nuclear Magnetic Resonance (NMR) has already proven to be a tremendous tool in –omics studies of Systems Biology, including the study of metabolome in biological systems known as metabolomics. Its major weakness is the low detection sensitivity that renders the analysis of microscopic quantities (submilligram) impractical, time consuming and often impossible. A cost-effective method is the use of micro(µ)-coils in NMR detections; however, implementing a NMR µcoil for heterogeneous samples such as tissues, cells and organisms is a challenging task. This is because of the necessity of spinning the sample at a 54.74° to the NMR magnetic field. This technique is known as Magic-Angle Spinning (MAS). The use of a spinning µcoil called High Resolution Magic Angle Coil Spinning (HRMACS) – developed by Alan Wong and his team at LSDRM marks the first successful µMAS NMR analyses of microscopic biospecimens, together with different biological expert-teams (INMG, ISA and CRMSB). Unfortunately HRMACS is greatly hindered by the impractical operation and the instability of µcoil. In 2015 without any financial supports, LSDRM has teamed up with a NMR-industry JEOL and developed an alternative technology, a standalone HRµMAS probe, specifically targeted to metabolomic applications. Unlike HRMACS, HRµMAS offers good metabolic analytical stability. However, further technological development and experimental validations are absolutely necessary in order for HRµMAS to have an impact in metabolomics. The aim of HRmicroMAS project is to implement and establish a truly convenient and accessible HRµMAS NMR-based application to the field of metabolomics. The project includes designing and constructing a stable HRµMAS probe, benchmarking and validating the HRµMAS NMR-based studies, and demonstrating its utilities with real applications. The success of the project will have a significant impact to the current NMR-platform for metabolomics, because it will be the first cornerstone of µMAS NMR-based metabolomics for microscopic specimens. The first half of the project will be dedicated to designing and building a reliable HRµMAS probe, and to benchmarking and validating the HRµMAS NMR experiments from sample-preparations to data acquisitions. The goal here is to develop a reliable HRµMAS NMR application that is convenient to everyone. The second half will carry out two independent pilot metabolomic studies with different teams whose are experts in metabolomic studies: (1) with the teams INMG and ISA, an metabolic investigation will be carried out on the heterogeneity of phenotype associated to aging within isogenic populations of Caenorhabditis elegans nematodes; and (2) with CRMSB, the metabolic profile of the brain energy metabolism in rat will be explored. These biological studies are designed to exploit and evaluate the different bioapplications of HRµMAS with different biological specimens, and will be an important milestone for HRmicroMAS, because it will demonstrate the wide utility of this innovative technology for NMR applications to in the field of metabolomics. HRmicroMAS fits well within the societal challenge #4 of “Life, Health and Well-Being” because it will be a vehicle that will lead to potential new discoveries and innovations in biological and medical science. And the strong collaborative nature in the multidisciplinary this project suits under the PRC financial instrument. It gathers µMAS experts (from LSDRM and JEOL), NMR spectroscopists (LSDRM and ISA) and biologists (INMG and CRMSB). This project also capitalizes a strong collaboration with a NMR industry, JEOL, whom will offer their involvements (probe design and construction) at zero-cost to the project. For this reason, HRmicroMAS can be considered a low-cost project (a requested fund of 304 k€) for a development of an innovative NMR application that could advance the analytical platform in metabolomics and open many new exciting venues.