The development of functional bioinspired polymer membranes is challenging due to the delicate balance needed for the membrane protein assembly, stability and function, which relies on specific and heterogeneous lipid environments. BIOMEM-Hop on aims to identify the precise lipid composition in newly developed biomimetic membranes required to maintain the structural integrity and activity of target transport proteins. BIOMEM-Hop on will implement an advanced high throughput (Epi)Lipidomics platform in synergy with bifunctional polymers testing, membrane kinetics, and transport activity assessments. This approach will significantly enhance the identification of specific lipid domains required for the stability and function of target transport proteins in protein-lipid domains and in biomimetic membranes. It will also shed light on how key lipids interact with bifunctional polymers, guiding the rational selection of polymers for the efficient extraction of active transport proteins along with their associated lipids. In addition, BIOMEM-Hop on aims to guarantee the uptake of its outcomes by the scientific community, stakeholders, policy makers and citizens to significantly increase the knowledge of the environmental, economic and societal benefits of this cost-effective, low-energy separation technique, as well as to explore additional opportunities in biomedicine and human healthcare. This will be achieved through communication, dissemination and exploitation of the research outputs. BIOMEM-Hop on is implemented by a strong consortium with expertise in polymer testing, transport protein stability and activity, membrane organization, kinetics and transport, and lipid analysis, laying the groundwork to understand and address the specific lipid composition required both for membrane protein activity and functional stability.