In silico i in vivo analiza transkriptoma stanične stijenke kvasca Saccharomyces cerevisiae i primjena dobivenih rezultata u konstrukciji novih biotehnoloških sojeva
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The development of the latest sequencing techniques has revealed an unexpectedly high complexity of the transcriptome in eukaryotic cells. Transcription occurring outside defined gene regions results in the emergence of non-coding RNAs (ncRNAs). With this project, we aim to shed light on the still underestimated impact and significance of ncRNAs in regulating proteins in the cell wall of the yeast Saccharomyces cerevisiae. We plan to conduct a bioinformatic meta-analysis and an in vivo analysis of the cell wall transcriptome to gather information about transcripts potentially regulated by non-coding RNAs under different conditions, at different growth stages, or during the cell cycle (mitosis and meiosis). This work should yield information that will enable us to construct new yeast strains with modified cell wall properties, which could, in turn, enhance various biotechnological processes.
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