Primjena proteinskih hidrolizata iz pogača lana i konoplje u medijima za uzgoj životinjskih stanica
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Oil-cakes of flax and industrial hemp seeds contain 30-35% proteins, and as such can be used as a nutritious media supplement for animal cell cultivation. The main objective of the proposed project is to determine the effects of flaxseed and hempseed oil cake hydrolysates on cell proliferation and recombinant protein production. We want to carry out an enzyme based oil cake hydrolysis and apply different hydrolysate fractions and concentrations in cell culture, with or without serum. Defining the parameters of hydrolysis and characterization of derived peptides is the foundation for standardization of the hydrolysate composition and their administration as nutritive supplements for cell culture. The results obtained with this project will contribute to the re-evaluation of waste-products from flax and hemp oil industry and direct their application in the field of animal technology. If positive results were achieved, (i.e. hydrolysates show stimulatory effects on cell growth and productivity), the selected hydrolysate fractions could be used as a beneficial culture media component and thus affect future media formulations.
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