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Publication . Article . 2011

Imaging of size-dependent uptake and identification of novel pathways in mouse Peyer's patches using fluorescent organosilica particles

Aziz Awaad; Michihiro Nakamura; Kazunori Ishimura;
Closed Access
Published: 29 Apr 2011 Journal: Nanomedicine: Nanotechnology, Biology and Medicine, volume 8, pages 627-636 (issn: 1549-9634, Copyright policy )
Publisher: Elsevier BV

Abstract We investigated size-dependent uptake of fluorescent thiol-organosilica particles by Peyer's patches (PPs). We performed an oral single-particle administration (95, 130, 200, 340, 695 and 1050 nm) and a simultaneous dual-particle administration using 2 kinds of particles. Histological imaging and quantitative analysis revealed that particles taken up by the PP subepithelial dome were size dependent, and there was an optimal size range for higher uptake. Quantitative analysis of simultaneous dual-particle administration revealed that the percentage of fluorescence areas for 95, 130, 200, 340, 695 and 1050 nm with respect to 110 nm area was 124.0, 89.1, 73.8, 20.2, 9.2 and 0.5%, respectively. Additionally, imaging using fluorescent thiol-organosilica particles could detect 2 novel pathways through mouse PP epithelium: the transcellular pathway and the paracellular pathway. The uptake of nanoparticles based on an optimal size range and 2 novel pathways could indicate a new approach for vaccine delivery and nanomedicine development. From the Clinical Editor Studying various sizes of fluorescent organosilica particles and their uptake in Peyer’s patches, this team of authors determined the optimal size range of administration. Two novel pathways through mouse Peyer’s patch epithelium were detected, i.e., the transcellular pathway and the paracellular pathway. This observation may have important applications in future vaccine delivery and nano-drug delivery.

Subjects by Vocabulary

Microsoft Academic Graph classification: Paracellular transport Materials science Particle size Epithelium medicine.anatomical_structure medicine Transcellular Nanoparticle Immunology Fluorescence Nanomedicine Biophysics Quantitative analysis (chemistry)


Pharmaceutical Science, General Materials Science, Biomedical Engineering, Molecular Medicine, Medicine (miscellaneous), Bioengineering, Administration, Oral, Animals, Dose-Response Relationship, Drug, Drug Delivery Systems, Fluorescence, Mice, Nanoparticles, Organosilicon Compounds, Particle Size, Peyer's Patches, Sulfhydryl Compounds, Vaccines

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