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One-pot synthesis and characterization of dual fluorescent thiol-organosilica nanoparticles as non-photoblinking quantum dots and their applications for biological imaging
Copyright policy )A multifunctional nanoparticle for biological applications has been developed by combining the useful functions of quantum dots (QDs), fluorescent dyes, and organosilica particles. Dual fluorescent thiol-organosilica nanoparticles composed of QDs and a fluorescent dye in a thiol-organosilica layer were synthesized in a one-pot process. The thiol-organosilica layer encapsulating the QDs could be tuned in thickness and functionalized. The QDs encapsulated with thiol-organosilica layer (thiol-OS-QDs) were well dispersed, and exhibited unique photophysical properties upon encapsulation within the thiol-organosilica layer. The thiol-OS-QDs could preserve their fluorescent properties under severe acid and alkali conditions as compared with those of bare QDs. The dual fluorescent thiol-OS-QDs containing QD605 and rhodamine B showed strong fluorescence, high photostability, and reduced photoblinking. Cells labeled with dual fluorescent thiol-OS-QDs were detected clearly in vivo, and their functionalization with antibody using the thiol residue on the surface was useful for molecular tracing of tumor antigen. The dual fluorescent thiol-OS-QDs showed high potential as non-photoblinking QDs.
Microsoft Academic Graph classification: Fluorescence One-pot synthesis Biological imaging Nanotechnology Thiol chemistry.chemical_classification chemistry Surface modification Nanoparticle Rhodamine B chemistry.chemical_compound Quantum dot Materials science
Medical Subject Headings: technology, industry, and agriculture equipment and supplies
Materials Chemistry, General Chemistry
Materials Chemistry, General Chemistry
Microsoft Academic Graph classification: Fluorescence One-pot synthesis Biological imaging Nanotechnology Thiol chemistry.chemical_classification chemistry Surface modification Nanoparticle Rhodamine B chemistry.chemical_compound Quantum dot Materials science
Medical Subject Headings: technology, industry, and agriculture equipment and supplies
- University of Tokushima Japan
- University of Tokushima Japan
A multifunctional nanoparticle for biological applications has been developed by combining the useful functions of quantum dots (QDs), fluorescent dyes, and organosilica particles. Dual fluorescent thiol-organosilica nanoparticles composed of QDs and a fluorescent dye in a thiol-organosilica layer were synthesized in a one-pot process. The thiol-organosilica layer encapsulating the QDs could be tuned in thickness and functionalized. The QDs encapsulated with thiol-organosilica layer (thiol-OS-QDs) were well dispersed, and exhibited unique photophysical properties upon encapsulation within the thiol-organosilica layer. The thiol-OS-QDs could preserve their fluorescent properties under severe acid and alkali conditions as compared with those of bare QDs. The dual fluorescent thiol-OS-QDs containing QD605 and rhodamine B showed strong fluorescence, high photostability, and reduced photoblinking. Cells labeled with dual fluorescent thiol-OS-QDs were detected clearly in vivo, and their functionalization with antibody using the thiol residue on the surface was useful for molecular tracing of tumor antigen. The dual fluorescent thiol-OS-QDs showed high potential as non-photoblinking QDs.