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Publication . Article . 2011

One-pot synthesis and characterization of dual fluorescent thiol-organosilica nanoparticles as non-photoblinking quantum dots and their applications for biological imaging

Michihiro Nakamura; Shuji Ozaki; Masahiro Abe; Toshio Matsumoto; Kazunori Ishimura;
Published: 01 Jan 2011 Journal: Journal of Materials Chemistry, volume 21, page 4,689 (issn: 0959-9428, eissn: 1364-5501, Copyright policy )
Publisher: Royal Society of Chemistry (RSC)

A multifunctional nanoparticle for biological applications has been developed by combining the useful functions of quantum dots (QDs), fluorescent dyes, and organosilica particles. Dual fluorescent thiol-organosilica nanoparticles composed of QDs and a fluorescent dye in a thiol-organosilica layer were synthesized in a one-pot process. The thiol-organosilica layer encapsulating the QDs could be tuned in thickness and functionalized. The QDs encapsulated with thiol-organosilica layer (thiol-OS-QDs) were well dispersed, and exhibited unique photophysical properties upon encapsulation within the thiol-organosilica layer. The thiol-OS-QDs could preserve their fluorescent properties under severe acid and alkali conditions as compared with those of bare QDs. The dual fluorescent thiol-OS-QDs containing QD605 and rhodamine B showed strong fluorescence, high photostability, and reduced photoblinking. Cells labeled with dual fluorescent thiol-OS-QDs were detected clearly in vivo, and their functionalization with antibody using the thiol residue on the surface was useful for molecular tracing of tumor antigen. The dual fluorescent thiol-OS-QDs showed high potential as non-photoblinking QDs.

Subjects by Vocabulary

Microsoft Academic Graph classification: Fluorescence One-pot synthesis Biological imaging Nanotechnology Thiol chemistry.chemical_classification chemistry Surface modification Nanoparticle Rhodamine B chemistry.chemical_compound Quantum dot Materials science

Medical Subject Headings: technology, industry, and agriculture equipment and supplies


Materials Chemistry, General Chemistry

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