Abstract An observing system simulation experiment (OSSE) has been carried out to evaluate the impact of a hybrid ensemble–variational data assimilation algorithm for use with the National Centers for Environmental Prediction (NCEP) global data assimilation system. An OSSE provides a controlled framework for evaluating analysis and forecast errors since a truth is known. In this case, the nature run was generated and provided by the European Centre for Medium-Range Weather Forecasts as part of the international Joint OSSE project. The assimilation and forecast impact studies are carried out using a model that is different than the nature run model, thereby accounting for model error and avoiding issues with the so-called identical-twin experiments. It is found that the quality of analysis is improved substantially when going from three-dimensional variational data assimilation (3DVar) to a hybrid 3D ensemble–variational (EnVar)-based algorithm. This is especially true in terms of the analysis error reduction for wind and moisture, most notably in the tropics. Forecast impact experiments show that the hybrid-initialized forecasts improve upon the 3DVar-based forecasts for most metrics, lead times, variables, and levels. An additional experiment that utilizes 3DEnVar (100% ensemble) demonstrates that the use of a 25% static error covariance contribution does not alter the quality of hybrid analysis when utilizing the tangent-linear normal mode constraint on the total hybrid increment.