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Research data . Dataset . 2019

Chlorophyll pigments and total organic carbon measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic

Kiesel, Joshua; Bienhold, Christina; Link, Heike;
Open Access
English
Published: 01 Jan 2019
Publisher: PANGAEA
Abstract

Chlorophyll pigments: Subsamples of the top first cm of sediment were obtained using cut-off plastic syringes. Samples for chlorophyll pigment analyses were stored dark and frozen at -20°C until further analyses in the home laboratory. Chlorophyll pigments were extracted as described in Boetius et al. (1998, https://doi.org/10.1016/S0967-0637(97)00052-6). Briefly, pigments were extracted in 90% acetone, after addition of glass beads and grinding in a cell mill. Extracts were centrifuged and concentrations of chlorophyll a and phaeopigments (after acidification with HCl) were determined with a Turner Trilogy fluorometer. The sum of chlorophyll a and phaeopigments is expressed as chloroplast pigment equivalents (CPE). The proportion of chlorophyll a from CPE (% chla) is an indicator of the freshness of phytodetritus. Total organic carbon: Subsamples of the top first cm of sediment were also obtained using cut-off plastic syringes. Samples were stored frozen at -20°C until further analyses in the home laboratory. The total carbon content of sediment samples was determined by combustion and gas chromatography with a Carlo Erba NA-1500 CNS elemental analyzer. An aliquot of the sample was acidified to remove the fraction of inorganic carbon and to determine the total amount of organic carbon.

Subjects

Assessment of bacterial life and matter cycling in deep sea surface sediments ABYSS, Barents Sea, Carbon, Chlorophyll a chloroplastic pigment equivalents ratio, Chlorophyll a per unit volume of sediment, Chlorophyll pigment equivalents, Comment, DEPTH, Date Time of event, Elevation of event, Event label, Gas chromatography with Carlo Erba NA 1500 CNS elemental analyzer, Giant box corer, Giant box grab, Latitude of event, Longitude of event, MultiCorer, PS94, Phaeopigments per unit volume of sediment, Polarstern, Sediment oxygen uptake, System Laptev Sea Transdrift TRANSDRIFT, Turner Trilogy fluorometer, bottom max, central Arctic Ocean, climate change, microbial abundance, organic, organic matter, sediment rock, top min, total, Earth System Research, organic-matter, Date/Time of event, DEPTH, sediment/rock, Depth, top/min, Depth, bottom/max, Carbon, organic, total, Chlorophyll a/chloroplastic pigment equivalents ratio, Gas chromatography with Carlo Erba NA-1500 CNS elemental analyzer, Assessment of bacterial life and matter cycling in deep-sea surface sediments (ABYSS), System Laptev-Sea: Transdrift (TRANSDRIFT)

Funded by
EC| ABYSS
Project
ABYSS
ABYSS - Assessment of bacterial life and matter cycling in deep-sea surface sediments
  • Funder: European Commission (EC)
  • Project Code: 294757
  • Funding stream: FP7 | SP2 | ERC
Related to Research communities
European Marine Science
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