You have already added 0 works in your ORCID record related to the merged Research product.
Delineating closely related dinoflagellate lineages using phylotranscriptomics
Recently radiated dinoflagellates Apocalathium aciculiferum (collected in Lake Erken, Sweden), Apocalathium malmogiense (Baltic Sea) and Apocalathium aff. malmogiense (Highway Lake, Antarctica) represent a lineage with an unresolved phylogeny. We determined their phylogenetic relationships using phylotranscriptomics based on 792 amino acid sequences. Our results showed that A. aciculiferum diverged from the other two closely related lineages, consistent with their different morphologies in cell size, relative cell length and presence of spines. We hypothesized that A. aff. malmogiense and A. malmogiense, which inhabit different hemispheres, are evolutionarily more closely related because they diverged from a marine common ancestor, adapting to a wide salinity range, while A. aciculiferum colonized a freshwater habitat, by acquiring adaptations to this environment, in particular, salinity intolerance. We show that phylotranscriptomics can resolve the phylogeny of recently diverged protists. This has broad relevance, given that many phytoplankton species are morphologically very similar, and single genes sometimes lack the information to determine species’ relationships
The work was supported through the Russian Foundation for Basic Research (project # 16-04-01704) to NA and a Swedish Research Council grant (2012-10-24) to KR as well as the marine microbial eukaryote transcriptome sequencing project (MMETSP) of the Gordon and Betty Moore Foundation
6 pages, 2 figures, supporting information https://doi.org/10.1111/jpy.12748
Peer Reviewed
Microsoft Academic Graph classification: Cytochrome b Biology Phylogenomics Phylogenetic tree Evolutionary biology Dinoflagellate biology.organism_classification Range (biology) Adaptive radiation Lineage (evolution) Phylogenetics
Medical Subject Headings: fungi
Internal transcribed spacer-2, ITS2, Cytochrome b, COB, Bootstrap support, BS, Protists, Adaptive radiation, Transcriptomes, Phylogenomics, Microalgae, High Throughput Sequencing, Dinoflagellida, Phylogeny, RNA, Algal, RNA, Protozoan, RNA, Ribosomal, Transcriptome, Plant Science, Aquatic Science, ta1181, ta1183, Internal transcribed spacer-2, ITS2, Cytochrome b, COB, Bootstrap support, BS, Protists, Adaptive radiation, Transcriptomes, Phylogenomics, Microalgae, High Throughput Sequencing
Internal transcribed spacer-2, ITS2, Cytochrome b, COB, Bootstrap support, BS, Protists, Adaptive radiation, Transcriptomes, Phylogenomics, Microalgae, High Throughput Sequencing, Dinoflagellida, Phylogeny, RNA, Algal, RNA, Protozoan, RNA, Ribosomal, Transcriptome, Plant Science, Aquatic Science, ta1181, ta1183, Internal transcribed spacer-2, ITS2, Cytochrome b, COB, Bootstrap support, BS, Protists, Adaptive radiation, Transcriptomes, Phylogenomics, Microalgae, High Throughput Sequencing
Microsoft Academic Graph classification: Cytochrome b Biology Phylogenomics Phylogenetic tree Evolutionary biology Dinoflagellate biology.organism_classification Range (biology) Adaptive radiation Lineage (evolution) Phylogenetics
Medical Subject Headings: fungi
- Finnish Environment Institute Finland
- Spanish National Research Council Spain
- Russian Academy of Sciences Russian Federation
- Lund University Sweden
- Finnish Environment Institute Finland
- Spanish National Research Council Spain
- Russian Academy of Sciences Russian Federation
- Lund University Sweden
Recently radiated dinoflagellates Apocalathium aciculiferum (collected in Lake Erken, Sweden), Apocalathium malmogiense (Baltic Sea) and Apocalathium aff. malmogiense (Highway Lake, Antarctica) represent a lineage with an unresolved phylogeny. We determined their phylogenetic relationships using phylotranscriptomics based on 792 amino acid sequences. Our results showed that A. aciculiferum diverged from the other two closely related lineages, consistent with their different morphologies in cell size, relative cell length and presence of spines. We hypothesized that A. aff. malmogiense and A. malmogiense, which inhabit different hemispheres, are evolutionarily more closely related because they diverged from a marine common ancestor, adapting to a wide salinity range, while A. aciculiferum colonized a freshwater habitat, by acquiring adaptations to this environment, in particular, salinity intolerance. We show that phylotranscriptomics can resolve the phylogeny of recently diverged protists. This has broad relevance, given that many phytoplankton species are morphologically very similar, and single genes sometimes lack the information to determine species’ relationships
The work was supported through the Russian Foundation for Basic Research (project # 16-04-01704) to NA and a Swedish Research Council grant (2012-10-24) to KR as well as the marine microbial eukaryote transcriptome sequencing project (MMETSP) of the Gordon and Betty Moore Foundation
6 pages, 2 figures, supporting information https://doi.org/10.1111/jpy.12748
Peer Reviewed