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Research data . Dataset . 2019

Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic

Bienhold, Christina;
Open Access
Published: 01 Jan 2019
Publisher: PANGAEA

For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged.


Barents Sea, central Arctic Ocean, climate change, microbial abundance, organic-matter, Sediment oxygen uptake, Event label, Date/Time of event, Latitude of event, Longitude of event, Elevation of event, DEPTH, sediment/rock, Depth, top/min, Depth, bottom/max, Prokaryotes, abundance as single cells, Comment, MultiCorer, Giant box grab, Giant box corer, Acridine Orange Direct Counting (AODC), PS94, Polarstern, Assessment of bacterial life and matter cycling in deep-sea surface sediments (ABYSS), System Laptev-Sea: Transdrift (TRANSDRIFT), Acridine Orange Direct Counting AODC, Assessment of bacterial life and matter cycling in deep sea surface sediments ABYSS, DEPTH, Date Time of event, Prokaryotes, System Laptev Sea Transdrift TRANSDRIFT, abundance as single cells, bottom max, organic matter, sediment rock, top min, Earth System Research

Funded by
ABYSS - Assessment of bacterial life and matter cycling in deep-sea surface sediments
  • Funder: European Commission (EC)
  • Project Code: 294757
  • Funding stream: FP7 | SP2 | ERC
Related to Research communities
European Marine Science